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FISH in Suspension or in Adherent Cells

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Fluorescence In-Situ Hybridization (FISH) for Microbial Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2246))

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Abstract

Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples.

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Acknowledgements

This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UIDB/50011/2020 & UIDP/50011/2020) and LAQV-Associate Laboratory for Green Chemistry (Ref. FCT UIDB/50006/2020), financed by national funds through the FCT/MCTES, and when appropriate cofinanced by FEDER under the PT2020 Partnership Agreement. Paulo C. Lemos acknowledges the support of FCT/MCTES for contract IF/01054/2014/CP1224/CT0005. Diogo Queirós and Joana Pereira thank FCT/MCTES for their PhD grants, SFRH/BD/87758/2012 and SFRH/BD/130003/2017, respectively.

Author information

Authors and Affiliations

  1. Water Research Institute C.N.R., Monterotondo, Italy

    Francesca Di Pippo & Simona Rossetti

  2. CICECO – Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal

    Diogo Queirós, Joana Pereira & Luísa S. Serafim

  3. LAQV, REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica, Portugal

    Paulo C. Lemos

Authors
  1. Francesca Di Pippo
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  2. Diogo Queirós
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  3. Joana Pereira
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  4. Paulo C. Lemos
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  5. Luísa S. Serafim
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  6. Simona Rossetti
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Corresponding author

Correspondence to Luísa S. Serafim .

Editor information

Editors and Affiliations

  1. LEPABE – Laboratory for Process Engineering Environment, Biotechnology and Energy, Department of Chemical Engineering, University of Porto, Porto, Portugal

    Nuno F. Azevedo

  2. INIAV – National Institute for Agrarian and Veterinarian Research, Rua dos Lagidos, Lugar da Madalena, Vairão, CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, LEPABE – Laboratory for Process Engineering Environment, Biotechnology and Energy, Department of Chemical Engineering, University of Porto, Vila do Conde, Portugal

    Carina Almeida

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Di Pippo, F., Queirós, D., Pereira, J., Lemos, P.C., Serafim, L.S., Rossetti, S. (2021). FISH in Suspension or in Adherent Cells. In: Azevedo, N.F., Almeida, C. (eds) Fluorescence In-Situ Hybridization (FISH) for Microbial Cells. Methods in Molecular Biology, vol 2246. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1115-9_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1115-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1114-2

  • Online ISBN: 978-1-0716-1115-9

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