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Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement

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Rice Genome Engineering and Gene Editing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2238))

Abstract

CRISPR-Cas resonates a revolutionary genome editing technology applicable through a horizon spreading across microbial organism to higher plant and animal. This technology can be harnessed with ease to understand the basic genetics of a living system by altering sequence of individual genes and characterizing their functions. The precision of this technology is unparallel. It allows very precise and targeted base pair level edits in the genome. Here, in the current chapter, we have provided end-to-end process outline on how to generate genome edited plants in crops like rice to evaluate for agronomic traits associated with yield, disease resistance and abiotic stress tolerance, etc. Genome editing process includes designing of gene editing strategy, vector construction, plant transformation, molecular screening, and phenotyping under control environment conditions. Furthermore, its application for development of commercial crop product may require additional processes, including field trials in the target geography for evaluation of product efficacy. Evaluation of genome edited lines in controlled greenhouse/net house or open field condition requires few generations for outcrossing with wild-type parent to eliminate and/or reduce any potential pleiotropic effect in the edited genome which may arise during the process. The genome edited plant selected for advancement shall harbor the genome with only the intended changes, which can be analyzed by various molecular techniques, advanced sequencing methods, and genomic data analysis tools. CRISPR-Cas-based genome editing has opened a plethora of opportunities in agriculture as well as human health.

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Acknowledgments

The work was carried out at the DuPont Knowledge Centre, Corteva Agriscience™, Hyderabad, India. We’d like to thank the senior leadership of Corteva for their guidance and support. The authors would also like to thank Dharmendra Patil, Syam Sura, Geeta Pogula, Nilesh Sapkal, Girish Chandra, and Sunder Reddy for their diligent work. The support of the Applied Science and Technology (AST) group of Corteva for molecular analysis of the edited lines is duly acknowledged. The options expressed in this manuscript are of the authors and not that of the Corteva Agriscience™.

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Authors and Affiliations

  1. Formerly at Corteva Agriscience™, DuPont Knowledge Centre, Hyderabad, Telangana, India

    Amit Das, Pallavi Ghana, Bhojaraja Rudrappa, Rita Gandhi, Venkata Sresty Tavva & Amitabh Mohanty

  2. Corteva Agriscience , Johnston, IA-50131, USA

    Amitabh Mohanty

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  1. Amit Das
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  2. Pallavi Ghana
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  3. Bhojaraja Rudrappa
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  4. Rita Gandhi
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  5. Venkata Sresty Tavva
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  6. Amitabh Mohanty
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Editor information

Editors and Affiliations

  1. Synthetic Biology, Biofuel and Genome Editing R&D, Reliance Industries Ltd, Navi Mumbai, Maharashtra, India

    Anindya Bandyopadhyay

  2. Crop Improvement and Genetics Research Unit, USDA-ARS, Albany, CA, USA

    Roger Thilmony

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Das, A., Ghana, P., Rudrappa, B., Gandhi, R., Tavva, V.S., Mohanty, A. (2021). Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement. In: Bandyopadhyay, A., Thilmony, R. (eds) Rice Genome Engineering and Gene Editing. Methods in Molecular Biology, vol 2238. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1068-8_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1068-8_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1067-1

  • Online ISBN: 978-1-0716-1068-8

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