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Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice

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Rice Genome Engineering and Gene Editing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2238))

Abstract

CRISPR-Cas9 and Cas12a (formerly Cpf1), RNA-guided DNA endonucleases found from adaptive immune system in prokaryotes, have been engineered and widely adopted as two of the most powerful genome editing systems in plants. Recently, we developed a single transcript unit (STU) CRISPR 2.0 toolbox for applications in plants, which contains two STU-Cas9 systems and one STU-Cas12a system. Here, we describe a detailed protocol about using the STU CRISPR 2.0 systems to achieve single and multiplex genome editing in rice.

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Acknowledgments

This work was supported by grants to Y.Z. from the Sichuan Youth Science and Technology Foundation (2017JQ0005), the National Science Foundation of China (31771486), the National Transgenic Major Project (2018ZX08022001-003), and the Fundamental Research Funds for the Central Universities (ZYGX2016J119 and ZYGX2016J122). This work was also partly supported by grants to Y.Q. from NSF (IOS-1758745), USDA-NIFA (2018-33522-28789), FFAR (593603), and Syngenta Biotechnology.

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Authors and Affiliations

  1. Department of Biotechnology, Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

    Xu Tang & Yong Zhang

  2. Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA

    Yiping Qi

  3. Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, USA

    Yiping Qi

Authors
  1. Xu Tang
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  2. Yiping Qi
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  3. Yong Zhang
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Corresponding author

Correspondence to Yong Zhang .

Editor information

Editors and Affiliations

  1. Synthetic Biology, Biofuel and Genome Editing R&D, Reliance Industries Ltd, Navi Mumbai, Maharashtra, India

    Anindya Bandyopadhyay

  2. Crop Improvement and Genetics Research Unit, USDA-ARS, Albany, CA, USA

    Roger Thilmony

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Tang, X., Qi, Y., Zhang, Y. (2021). Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice. In: Bandyopadhyay, A., Thilmony, R. (eds) Rice Genome Engineering and Gene Editing. Methods in Molecular Biology, vol 2238. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1068-8_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1068-8_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1067-1

  • Online ISBN: 978-1-0716-1068-8

  • eBook Packages: Springer Protocols

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