Abstract
The sandwich-based immunoassay is renowned for its specificity and sensitivity for protein detection, where the antigen is “sandwiched” by a pair of antigen epitope-specific antibodies. The capture antibody-antigen-detection antibody complex will be formed if all the components are present in a proper reaction system. A one-step rapid sandwich-based antibody array can be developed through fixing the capture antibody on a glass slide with a fluorescence-labelled detection antibody. In this chapter, we describe the process of a one-step mouse immunoglobulin isotyping array for use in hybridoma culture supernatants.
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Mao, Y.Q., Li, LH. (2021). One-Step Antibody Arrays. In: Whittaker, K.C., Huang, RP. (eds) Antibody Arrays. Methods in Molecular Biology, vol 2237. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1064-0_3
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DOI: https://doi.org/10.1007/978-1-0716-1064-0_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1063-3
Online ISBN: 978-1-0716-1064-0
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