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Self-Assembling Peptide Hydrogels for 3D Microarrays

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2237)

Abstract

Recent advances in biosensing analytical platforms have brought relevant outcomes for novel diagnostic and therapy-oriented applications. In this context, hydrogels have emerged as appealing matrices to locally confine biomolecules onto sensing surfaces under solution mimetic conditions, preserving their structural integrity and function. Here, we describe the application of a self-assembling peptide hydrogel as a suitable matrix for 3D microarray bioassays. The hydrogel is printable and self-adhesive and allows for fast analyte diffusion. As a showcase example, we describe its application in a diagnostic immunoassay for the detection of arbovirus infection.

Key words

Self-assembly peptide hydrogels 3D bioassays Supramolecular nanostructures Microarrays 
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Notes

Acknowledgments

Fondazione Cariplo and Regione Lombardia, project HYDROGEX are gratefully acknowledged for financial support (grant n. 2018-1720). This work was also partially funded by Project READY (Regione Lombardia, Grant ID 229472).

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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.National Research Council of Italy—Istituto di Scienze e Tecnologie Chimiche (SCITEC-CNR)MilanItaly

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