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In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function

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Myeloid-Derived Suppressor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2236))

Abstract

Myeloid-derived suppressor cells (MDSC) appear at relatively low frequencies in diseased organs such as tumors or infection sites, but accumulate systemically in the spleen. So far MDSC have been reported in humans and experimental animals such as mice, rats, and nonhuman primates. Therefore, methods to generate MDSC in large amounts in vitro can serve as an additional tool to study their biology. Here, we describe in detail the generation of murine MDSC with GM-CSF from bone marrow (BM). Both subsets of granulocytic (G-MDSC) and monocytic MDSC (M-MDSC) are generated by this cytokine. We provide panels of phenotypic markers to distinguish them from non-suppressive cells and define developmental stages of monocytes developing into M-MDSC by two subsequent steps in vitro.

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Acknowledgments

The research work of our lab on MDSC has been supported by funding through grants from DFG under LU851/6-2 and 18-1.

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Authors and Affiliations

  1. Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany

    Ina Eckert, Eliana Ribechini & Manfred B. Lutz

Authors
  1. Ina Eckert
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  2. Eliana Ribechini
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  3. Manfred B. Lutz
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Corresponding author

Correspondence to Manfred B. Lutz .

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Editors and Affiliations

  1. Experimental and Translational Research, Department of Otorhinolaryngology, University Hospital Essen, West German Cancer Center, Essen, Germany

    Sven Brandau

  2. Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany

    Anca Dorhoi

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Eckert, I., Ribechini, E., Lutz, M.B. (2021). In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function. In: Brandau, S., Dorhoi, A. (eds) Myeloid-Derived Suppressor Cells. Methods in Molecular Biology, vol 2236. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1060-2_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1060-2_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1059-6

  • Online ISBN: 978-1-0716-1060-2

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