Abstract
The microbial cellulase system is responsible for the generation of glucose from cellulose. Cellulases are comprised of at least three major groups of enzymes, namely endoglucanases, exoglucanases, and β-glucosidases. On the other hand, xylanases function in the degradation of hemicellulose and work synergistically with cellulases for the degradation of lignocellulosic biomass. Here, we describe the most commonly used methods for the activity measurement of cellulases and xylanases.
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References
Zhang F, Bunterngsook B, Li J-X, Zhao X-Q, Champreda V, Liu C-G, Bai F-W (2019) Chapter 3 - Regulation and production of lignocellulolytic enzymes from Trichoderma reesei for biofuels production. In: Li Y, Ge X (eds) Advances in bioenergy, vol 4. Elsevier, Cambridge, MA, pp 79–119. https://doi.org/10.1016/bs.aibe.2019.03.001
Wang M, Li Z, Fang X, Wang L, Qu Y (2012) Cellulolytic enzyme production and enzymatic hydrolysis for second-generation bioethanol production. In: Bai F-W, Liu C-G, Huang H, Tsao GT (eds) Biotechnology in China III: biofuels and bioenergy. Springer, Berlin, pp 1–24. https://doi.org/10.1007/10_2011_131
Mullings R (1985) Measurement of saccharification by cellulases. Enzyme Microb Technol 7(12):586–591. https://doi.org/10.1016/0141-0229(85)90025-0
Wood TM, Bhat KM (1988) Methods for measuring cellulase activities. Methods Enzymol 160:87–112. https://doi.org/10.1016/0076-6879(88)60109-1
Helbert W, Chanzy H, Husum TL, Schülein M, Ernst S (2003) Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity. Biomacromolecules 4(3):481–487. https://doi.org/10.1021/bm020076i
Eveleigh DE, Mandels M, Andreotti R, Roche C (2009) Measurement of saccharifying cellulase. Biotechnol Biofuels 2(1):21. https://doi.org/10.1186/1754-6834-2-21
Dashtban M, Maki M, Leung KT, Mao C, Qin W (2010) Cellulase activities in biomass conversion: measurement methods and comparison. Crit Rev Biotechnol 30(4):302–309. https://doi.org/10.3109/07388551.2010.490938
Ghose T (1987) Measurement of cellulase activities. Pure Appl Chem 59(2):257–268
Zhang YHP, Hong J, Ye X (2009) Cellulase Assays. In: Mielenz JR (ed) Biofuels: methods and protocols. Humana Press, Totowa, NJ, pp 213–231. https://doi.org/10.1007/978-1-60761-214-8_14
Jourdier E, Cohen C, Poughon L, Larroche C, Monot F, Chaabane FB (2013) Cellulase activity mapping of Trichoderma reesei cultivated in sugar mixtures under fed-batch conditions. Biotechnol Biofuels 6(1):79. https://doi.org/10.1186/1754-6834-6-79
Deshpande MV, Eriksson K-E, Göran Pettersson L (1984) An assay for selective determination of exo-1,4,-β-glucanases in a mixture of cellulolytic enzymes. Anal Biochem 138(2):481–487. https://doi.org/10.1016/0003-2697(84)90843-1
Percival Zhang YH, Himmel ME, Mielenz JR (2006) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24(5):452–481. https://doi.org/10.1016/j.biotechadv.2006.03.003
Coward-Kelly G, Aiello-Mazzari C, Kim S, Granda C, Holtzapple M (2003) Suggested improvements to the standard filter paper assay used to measure cellulase activity. Biotechnol Bioeng 82(6):745–749. https://doi.org/10.1002/bit.10620
Zhang YHP, Lynd LR (2005) Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis. Biomacromolecules 6(3):1510–1515. https://doi.org/10.1021/bm049235j
Zhang Y-HP, Lynd LR (2004) Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems. Biotechnol Bioeng 88(7):797–824. https://doi.org/10.1002/bit.20282
Zhang Y-HP, Lynd LR (2006) A functionally based model for hydrolysis of cellulose by fungal cellulase. Biotechnol Bioeng 94(5):888–898. https://doi.org/10.1002/bit.20906
Bhat KM, Hay AJ, Claeyssens M, Wood TM (1990) Study of the mode of action and site-specificity of the endo-(1-4)-beta-D-glucanases of the fungus Penicillium pinophilum with normal, 1-3H-labelled, reduced and chromogenic cello-oligosaccharides. Biochem J 266(2):371–378. https://doi.org/10.1042/bj2660371
Bhat S, Kennedy JF, Goodenough PW, Owen E, Bhat MK (1997) Effect of d-glucono-1,4-lactone on the production of CMCase, pNPCase and true cellulase by Clostridium thermocellum. Carbohyd Polym 34(1):95–99. https://doi.org/10.1016/S0144-8617(97)00049-0
Claeyssens M, Aerts G (1992) Characterisation of cellulolytic activities in commercial Trichoderma reesei preparations: an approach using small, chromogenic substrates. Bioresour Technol 39(2):143–146. https://doi.org/10.1016/0960-8524(92)90133-I
Ghose T, Bisaria VS (1987) Measurement of hemicellulase activities: part I xylanases. Pure Appl Chem 59(12):1739–1751
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This work was supported from the Natural Science Foundation of China (grant number 21536006).
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Meng, QS., Zhang, F., Liu, CG., Bai, FW., Zhao, XQ. (2021). Measurement of Cellulase and Xylanase Activities in Trichoderma reesei. In: Mach-Aigner, A.R., Martzy, R. (eds) Trichoderma reesei. Methods in Molecular Biology, vol 2234. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1048-0_12
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