Advertisement

Sampling Microbiomes Associated with Different Plant Compartments

Protocol
  • 1k Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2232)

Abstract

The microbiome is known to influence plant fitness and differs significantly between plant compartments. To characterize the communities associated with different plant compartments, it is necessary to separate plant tissues in a manner that is suitable for microbiome analysis. Here, we describe a standardized protocol for sampling the microbiomes associated with bulk soil, the apical and basal ectorhizosphere, the apical and ectorhizosphere, the rhizome, pseudostem, and leaves of Musa spp. The approach can easily be modified for work with other plants.

Key words

Sampling Microbiome Plant Sequencing Bacteria Fungi Rhizosphere 

References

  1. 1.
    Santoyo G, Moreno-Hagelsieb G, del Carmen Orozco-Mosqueda M, Glick BR (2016) Plant growth-promoting bacterial endophytes. Microbiol Res 183:92–99CrossRefGoogle Scholar
  2. 2.
    Kudoyarova GR, Vysotskaya LB, Arkhipova TN, Kuzmina LY, Galimsyanova NF, Sidorova LV et al (2017) Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiol Plant 39:1–8CrossRefGoogle Scholar
  3. 3.
    Berg M, Koskella B (2018) Nutrient- and dose-dependent microbiome-mediated protection against a plant pathogen. Curr Biol 28:2487–2492CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Kim Y-C, Glick BR, Bashan Y, Ryu C-M (2012) Enhancement of plant drought tolerance by microbes. In: Plant responses to drought stress. Springer, Berlin, p 383Google Scholar
  5. 5.
    Evelin H, Kapoor R, Giri B (2009) Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot 104:1263–1280CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Coleman-derr D, Desgarennes D, Fonseca-garcia C, Gross S, Clingenpeel S, Woyke Y et al (2016) Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species. New Phytol 209:798–811CrossRefPubMedGoogle Scholar
  7. 7.
    Watt M, Hugenholtz P, White R, Vinall K (2006) Numbers and locations of native bacteria on field-grown wheat roots quantified by fluorescence in situ hybridization (FISH). Environ Microbiol 8:871–884CrossRefPubMedGoogle Scholar
  8. 8.
    van Vuurde JWL, Schippers B (1980) Bacterial colonisation of seminal wheat roots. Soil Biol Biochem 12:559–565CrossRefGoogle Scholar
  9. 9.
    Olsson S, Baath E, Soderstrom B (1987) Growth of Verticillium dahliae Kleb. hyphae and of bacteria along the roots of rape (Brassica napus L.) seedlings. Can J Microbiol 39:916–919CrossRefGoogle Scholar
  10. 10.
    Parke JL, Moen R, Rovira AD, Bowen GD (1986) Soil water affects the rhizosphere distribution of a seed-borne biological control agent, Pseudomonas fluorescens. Soil Biol Biochem 18:583–588CrossRefGoogle Scholar
  11. 11.
    Deangelis KM, Brodie EL, DeSantis TZ, Andersen GL, Lindow SE, Firestone MK (2009) Selective progressive response of soil microbial community to wild oat roots. ISME J 3:168–178CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.School of Earth and Environmental SciencesThe University of QueenslandBrisbaneAustralia
  2. 2.Department of Agriculture and FisheriesCentre for Wet Tropic AgricultureSouth JohnstoneAustralia

Personalised recommendations