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Prediction of Cellular Burden with Host–Circuit Models

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Part of the Methods in Molecular Biology book series (MIMB, volume 2229)

Abstract

Heterologous gene expression draws resources from host cells. These resources include vital components to sustain growth and replication, and the resulting cellular burden is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host–circuit models to predict the impact of design parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modeling paradigm can facilitate fast and robust design cycles in synthetic biology.

Key words

Cellular burden Growth models Whole-cell modeling Gene circuit design Synthetic biology Resource allocation 

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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of EdinburghEdinburghUK
  2. 2.School of InformaticsUniversity of EdinburghEdinburghUK

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