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Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse

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Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2224))

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Abstract

Hematopoiesis in the mouse and other mammals occurs in several waves and arises from distinct anatomic sites. Transgenic mice expressing fluorescent reporter proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to more restricted progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene, survey available fluorescent probes, and describe methods for confirming and analyzing transgene expression in the hematopoietic tissues of the embryo, fetus, and postnatal/adult animal.

Jeffrey Barminko and Andrei M. Vacaru are Co-first authors.

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Acknowledgments

Work in our laboratory was supported in part by grants to M.H.B. from the National Institutes of Health (R01 HL62248 and DK52191). Jeffrey Barminko and Andrei M. Vacaru contributed equally to this work.

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Author notes

  1. Andrei M. Vacaru

    Present address: Institute for Cellular Biology and Pathology, Bucharest, Romania

Authors and Affiliations

  1. Department of Medicine, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Jeffrey Barminko & Andrei M. Vacaru

  2. Departments of Medicine, Cell, Developmental, and Regenerative Biology, and Oncological Sciences, The Tisch Cancer Institute, The Black Family Stem Cell Institute, and The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Margaret H. Baron

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Correspondence to Margaret H. Baron .

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Editors and Affiliations

  1. Basic Research Laboratory, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Surgery, University of California, AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  3. Department of Biology, University of Dayton, Dayton, OH, USA

    Amit Singh

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Barminko, J., Vacaru, A.M., Baron, M.H. (2021). Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse. In: Singh, S.R., Hoffman, R.M., Singh, A. (eds) Mouse Genetics . Methods in Molecular Biology, vol 2224. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1008-4_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1008-4_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1007-7

  • Online ISBN: 978-1-0716-1008-4

  • eBook Packages: Springer Protocols

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