Abstract
Colorectal cancer animal model is a very useful tool to explore the tumor initiation and development. In the past year, many methods have been used for building up the mouse model including the subcutaneous injection and cecal wall injection or implantation. But this model cannot reflect the native stromal environment of the colon mucosa. Recently, the in vivo murine endoscopy has been developed allowing high-resolution imaging of the colon. Endoscopy orthotopic injection tumor cell line becomes a low cost, fast tumor growth simple technique. In this chapter, we describe detailed protocols for rapidly and efficiently building up colon cancer tumors model by using the colonoscopy-guided mucosal injection. This model can be used to explore drug testing, gene function assessment, and cancer metastasis.
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Acknowledgments
This research was supported by an internal grant of the University of Ulsan, College of Medicine (Physician Scientist Program) and a grant from Asan Institute for Life Sciences (grant No.2019 IP0571).
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Chang, S. (2021). Generation of Colon Cancer Model Based on Colonoscopy Injection. In: Singh, S.R., Hoffman, R.M., Singh, A. (eds) Mouse Genetics . Methods in Molecular Biology, vol 2224. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1008-4_11
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DOI: https://doi.org/10.1007/978-1-0716-1008-4_11
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Online ISBN: 978-1-0716-1008-4
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