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Mouse Models of Colitis-Associated Colon Cancer

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Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2224))

Abstract

Crohn’s disease (CD) and ulcerative colitis are two main clinically defined forms of chronic inflammatory bowel disease (IBD). Chronic intestinal inflammation is inextricably linked to colitis-associated colon carcinogenesis (CAC). Patients with ulcerative colitis (UC) and Crohn’s disease (CD) have an increased risk of colon cancer. Our understanding of IBD and IBD-associated colon carcinogenesis depends largely on rodent models. AOM-DSS-induced colitis-associated colon cancer in mice is the most widely used and accepted model that can recapitulate the human IBD-associated colon cancer. Here, we have provided detailed protocols of this mouse model of experimentally induced chronic intestinal inflammation-associated colon cancer. We will also discuss the protocols for the isolation and analysis of inflammatory immune cells from the colon.

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Acknowledgments

Funding provided by NIH (R01DK097271, R01DK123360) and Augusta University institutional development funds.

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Authors and Affiliations

  1. Georgia Cancer Center, Augusta University, Augusta, GA, USA

    Santhakumar Manicassamy & Daniel Swafford

  2. Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA, USA

    Santhakumar Manicassamy & Puttur D. Prasad

  3. Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA

    Santhakumar Manicassamy

Authors
  1. Santhakumar Manicassamy
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  2. Puttur D. Prasad
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  3. Daniel Swafford
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Corresponding author

Correspondence to Santhakumar Manicassamy .

Editor information

Editors and Affiliations

  1. Basic Research Laboratory, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Surgery, University of California, AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  3. Department of Biology, University of Dayton, Dayton, OH, USA

    Amit Singh

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Manicassamy, S., Prasad, P.D., Swafford, D. (2021). Mouse Models of Colitis-Associated Colon Cancer. In: Singh, S.R., Hoffman, R.M., Singh, A. (eds) Mouse Genetics . Methods in Molecular Biology, vol 2224. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1008-4_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1008-4_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1007-7

  • Online ISBN: 978-1-0716-1008-4

  • eBook Packages: Springer Protocols

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