Advertisement

Control of Listeria monocytogenes Biofilms in a Simulated Food-Processing Environment

Protocol
  • 398 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2220)

Abstract

Biofilm-forming ability may vary significantly among different Listeria (L.) monocytogenes strains. This interstrain variation is also observed in L. monocytogenes biofilm resistance to antimicrobial compounds commonly used in the food-processing environment. The screening of a large set of L. monocytogenes strains with specific characteristics, such as serotype, MLST type, and other genetic characteristics under various environmental conditions, may lead to a better understanding of the mechanisms underlying the establishment of the pathogen on food contact surfaces. In this chapter, traditional methods for L. monocytogenes strains characterization with regard to biofilm formation and novel biofilm control methods will be described.

Key words

Biofilm Attachment Detachment Antimicrobial resistance Sanitizer Bead vortexing Crystal violet Food soil 

References

  1. 1.
    Renier S, Hébraud M, Desvaux M (2011) Molecular biology of surface colonization by Listeria monocytogenes: an additional facet of an opportunistic Gram-positive foodborne pathogen. Environ Microbiol 13:835–850.  https://doi.org/10.1111/j.1462-2920.2010.02378.xCrossRefPubMedGoogle Scholar
  2. 2.
    Todhanakasem T, Young GM (2008) Loss of flagellum-based motility by Listeria monocytogenes results in formation of hyperbiofilms. J Bacteriol 190:6030–6034.  https://doi.org/10.1128/JB.00155-08CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Nilsson RE, Ross T, Bowman JP (2011) Variability in biofilm production by Listeria monocytogenes correlated to strain origin and growth conditions. Int J Food Microbiol 150:14–24.  https://doi.org/10.1016/j.ijfoodmicro.2011.07.012CrossRefPubMedGoogle Scholar
  4. 4.
    Doijad SP, Barbuddhe SB, Garg S, Poharkar KV (2015) Biofilm-forming abilities of Listeria monocytogenes serotypes isolated from different sources. PLoS One.  https://doi.org/10.1371/journal.pone.0137046
  5. 5.
    Chavant P, Martinie B, Meylheuc T, Hebraud M (2002) Listeria monocytogenes LO28: surface physicochemical properties and ability to form biofilms at different temperatures and growth phases. Appl Environ Microbiol 68:728–737.  https://doi.org/10.1128/AEM.68.2.728CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Lemon KP, Freitag NE, Kolter R (2010) The virulence regulator PrfA promotes biofilm formation by Listeria monocytogenes. J Bacteriol 192:3969–3976.  https://doi.org/10.1128/JB.00179-10CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Poimenidou SV, Chrysadakou M, Tzakoniati A et al (2016) Variability of Listeria monocytogenes strains in biofilm formation on stainless steel and polystyrene materials and resistance to peracetic acid and quaternary ammonium compounds. Int J Food Microbiol 237:164–171.  https://doi.org/10.1016/j.ijfoodmicro.2016.08.029CrossRefPubMedGoogle Scholar
  8. 8.
    Kadam SR, Den Besten HMW, Van Der Veen S et al (2013) Diversity assessment of Listeria monocytogenes biofilm formation: impact of growth condition, serotype and strain origin. Int J Food Microbiol 165:259–264.  https://doi.org/10.1016/j.ijfoodmicro.2013.05.025CrossRefPubMedGoogle Scholar
  9. 9.
    Halberg Larsen M, Dalmasso M, Ingmer H et al (2014) Persistence of foodborne pathogens and their control in primary and secondary food production chains. Food Control 44:92–109.  https://doi.org/10.1016/j.foodcont.2014.03.039CrossRefGoogle Scholar
  10. 10.
    Ferreira V, Wiedmann M, Teixeira P, Stasiewicz MJ (2014) Listeria monocytogenes persistence in food-associated environments: epidemiology, strain characteristics, and implications for public health. J Food Prot 77:150–170.  https://doi.org/10.4315/0362-028X.JFP-13-150CrossRefPubMedGoogle Scholar
  11. 11.
    Carpentier B, Cerf O (2011) Review-Persistence of Listeria monocytogenes in food industry equipment and premises. Int J Food Microbiol 145:1–8.  https://doi.org/10.1016/j.ijfoodmicro.2011.01.005CrossRefPubMedGoogle Scholar
  12. 12.
    Lundén J, Autio T, Markkula A et al (2003) Adaptive and cross-adaptive responses of persistent and non-persistent Listeria monocytogenes strains to disinfectants. Int J Food Microbiol 82:265–272.  https://doi.org/10.1016/S0168-1605(02)00312-4CrossRefPubMedGoogle Scholar
  13. 13.
    Aarnisalo K, Lunden J, Korkeala H, Wirtanen G (2007) Susceptibility of Listeria monocytogenes strains to disinfectants and chlorinated alkaline cleaners at cold temperatures. LWT - Food Sci Technol 40:1041–1048.  https://doi.org/10.1016/j.lwt.2006.07.009CrossRefGoogle Scholar
  14. 14.
    To MS, Favrin S, Romanova N, Griffiths MW (2002) Postadaptational resistance to benzalkonium chloride and subsequent physicochemical modifications of Listeria monocytogenes. Appl Environ Microbiol 68:5258–5264.  https://doi.org/10.1128/AM.68.11.5258CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Gray JA, Chandry PS, Kaur M et al (2018) Novel biocontrol methods for Listeria monocytogenes biofilms in food production facilities. Front Microbiol 9:1–12.  https://doi.org/10.3389/fmicb.2018.00605CrossRefGoogle Scholar
  16. 16.
    Zilelidou EA, Skandamis PN (2018) Growth, detection and virulence of Listeria monocytogenes in the presence of other microorganisms: microbial interactions from species to strain level. Int J Food Microbiol 277:10–25.  https://doi.org/10.1016/j.ijfoodmicro.2018.04.011CrossRefPubMedGoogle Scholar
  17. 17.
    Strauch E, Hammerl JA, Hertwig S (2007) Bacteriophages: new tools for safer food? J Consum Prot Food Safety 2:138–143.  https://doi.org/10.1007/s00003-007-0188-5CrossRefGoogle Scholar
  18. 18.
    Guenther S, Huwyler D, Richard S, Loessner MJ (2009) Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods. Appl Environ Microbiol 75:93–100.  https://doi.org/10.1128/AEM.01711-08CrossRefPubMedGoogle Scholar
  19. 19.
    Stepanović S, Cirković I, Ranin L, Svabić-Vlahović M (2004) Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Lett Appl Microbiol 38:428–432.  https://doi.org/10.1111/j.1472-765X.2004.01513.xCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Agricultural University of AthensAthensGreece

Personalised recommendations