Skip to main content
SpringerLink
Log in
Book cover

Germline Development in the Zebrafish pp 253–263Cite as

Histological Analysis of Gonads in Zebrafish

Part of the Methods in Molecular Biology book series (MIMB,volume 2218)

Abstract

Zebrafish is an excellent system for the study of gonad development due to available genetic tools and its utilization as a human disease model. The zebrafish serves as an experimental system to model human disorders affecting the reproductive system, toxicological effects on fertility and sexual development, and hormonal regulation of fertility. Forward genetic screens have been used to uncover genetic causes of infertility and reverse genetic approaches have demonstrated that genes involved in germ cell development have similar functions in zebrafish and mammals. The most comprehensive picture of the gonad can be visualized by histology. There are a variety of methods that give excellent histology of zebrafish gonads. Below are methods for two staining approaches for the histology of paraffin-embedded zebrafish gonads.

Key words

  • Ovary
  • Testis
  • Histology
  • Germ cells

This is a preview of subscription content, access via your institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Leal MC, Cardoso ER, Nóbrega RH, Batlouni SR, Bogerd J, França LR et al (2009) Histological and stereological evaluation of zebrafish (Danio rerio) spermatogenesis with an emphasis on spermatogonial generations. Biol Reprod 81:177–187. https://doi.org/10.1095/biolreprod.109.076299

    CrossRef  CAS  PubMed  Google Scholar 

  2. Draper BW, Siegfried KR (2019) The reproductive system. In: Cartner SC, Eisen JS, Farmer SC, Guillemin KJ, Kent ML, Sanders GE (eds) The zebrafish in biomedical research: biology, husbandry, diseases, and research applications. Elsevier, Amsterdam

    Google Scholar 

  3. Selman K, Wallace RA, Sarka A, Qi X. Stages of oocyte development in the zebrafish, Brachydanio rerio,J Morphol 1993;218: 203–224. doi:https://doi.org/10.1002/jmor.1052180209

  4. Houwing S, Kamminga LM, Berezikov E, Cronembold D, Girard A, van den Elst H et al (2007) A rRole for Piwi and piRNAs in germ cell maintenance and transposon silencing in zebrafish. Cell 129:69–82. https://doi.org/10.1016/j.cell.2007.03.026

    CrossRef  CAS  PubMed  Google Scholar 

  5. Maack G, Segner H (2003) Morphological development of the gonads in zebrafish. J Fish Biol. https://doi.org/10.1046/j.1095-8649.2003.00074.x

  6. Rodríguez-Marí A, Wilson C, Titus TA, Cañestro C, BreMiller RA, Yan Y-L et al (2011) Roles of brca2 (fancd1) in oocyte nuclear architecture, gametogenesis, gonad tumors, and genome stability in zebrafish. PLoS Genet 7:e1001357. https://doi.org/10.1371/journal.pgen.1001357

    CrossRef  CAS  PubMed  PubMed Central  Google Scholar 

  7. Webster KA, Schach U, Ordaz A, Steinfeld JS, Draper BW, Siegfried KR (2017) Dmrt1 is necessary for male sexual development in zebrafish. Dev Biol 422:33–46. https://doi.org/10.1016/j.ydbio.2016.12.008

    CrossRef  CAS  PubMed  Google Scholar 

  8. Saito K, Siegfried KR, Nüsslein-Volhard C, Sakai N (2011) Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants. Dev Dyn 240:1779–1792. https://doi.org/10.1002/dvdy.22661

    CrossRef  CAS  PubMed  Google Scholar 

  9. Braat AK, Zandbergen T, Van De Water S, Goos HJT, Zivkovic D (1999) Characterization of zebrafish primordial germ cells: morphology and early distribution of vasa RNA. Dev Dyn 216:153–167. https://doi.org/10.1002/(SICI)1097-0177(199910)216:2<153::AID-DVDY6>3.0.CO;2-1

    CrossRef  CAS  PubMed  Google Scholar 

  10. Gupta T, Marlow FL, Ferriola D, Mackiewicz K, Dapprich J, Monos D et al (2010) Microtubule actin crosslinking factor 1 regulates the balbiani body and animal-vegetal polarity of the zebrafish oocyte. PLoS Genet 6:e1001073. https://doi.org/10.1371/journal.pgen.1001073

    CrossRef  CAS  PubMed  PubMed Central  Google Scholar 

  11. Köhler A, Collymore C, Finger-Baier K, Geisler R, Kaufmann L, Pounder KC et al (2017) Report of workshop on euthanasia for zebrafish – a matter of welfare and science. Zebrafish. https://doi.org/10.1089/zeb.2017.1508

Download references

Author information

Authors and Affiliations

  1. Biology Department, University of Massachusetts Boston, Boston, MA, USA

    Kellee R. Siegfried & Jocelyn S. Steinfeld

Authors
  1. Kellee R. Siegfried
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jocelyn S. Steinfeld
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kellee R. Siegfried .

Editor information

Editors and Affiliations

  1. Institute for Developmental Biochemistry, University of Göttingen Medical Center Georg-August-Universität, Göttingen, Germany

    Dr. Roland Dosch

Rights and permissions

Reprints and Permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Siegfried, K.R., Steinfeld, J.S. (2021). Histological Analysis of Gonads in Zebrafish . In: Dosch, R. (eds) Germline Development in the Zebrafish. Methods in Molecular Biology, vol 2218. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0970-5_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0970-5_20

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0969-9

  • Online ISBN: 978-1-0716-0970-5

  • eBook Packages: Springer Protocols

search

Navigation