Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads

Part of the Methods in Molecular Biology book series (MIMB, volume 2217)


Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein–protein interactions and signaling.

Key words

Focal adhesion composition Magnetics Mechanotransduction 


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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Wallenberg Centre for Molecular Medicine, Division of Oncology, Department of Clinical SciencesLund UniversityLundSweden

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