Abstract
The specification and development of germ cells to gametes is a unique process, which is of great biological and clinical relevance. In mammals, the founding cells of the germline are primordial germ cells (PGCs), which arise during early embryogenesis. The low number of PGCs within the developing embryo limits the study of these cells in model organisms. The generation of PGC-like cells (PGCLCs) from murine pluripotent stem cells reconstitutes the earliest stages of germ cell development and mitigates the technical constraints of studying this developmental process in vivo. Here, we describe the technical details of the PGCLC specification approach and illustrate adaptations designed to improve compatibility with methods such as chromatin immunoprecipitation by increasing the yield of PGCLC generation.
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Acknowledgements
This work was kindly supported by Professor Azim Surani and the Wellcome Trust Senior Investigator grant (RG92113) awarded to Professor Azim Surani. U.G. is supported by a Sofja Kovalevskaja Award of the Humboldt foundation.
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Gruhn, W.H., Günesdogan, U. (2021). Generation of Primordial Germ Cell-like Cells on Small and Large Scales. In: Ancelin, K., Borensztein, M. (eds) Epigenetic Reprogramming During Mouse Embryogenesis. Methods in Molecular Biology, vol 2214. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0958-3_6
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DOI: https://doi.org/10.1007/978-1-0716-0958-3_6
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