Abstract
Over the past two decades, the development of chromosome conformation capture technologies has allowed to intensively probe the properties of genome folding in various cell types. High-throughput versions of these C-based assays (named Hi-C) have released the mapping of 3D chromosome folding for the entire genomes. Applied to mammalian preimplantation embryos, it has revealed a unique chromosome organization after fertilization when a new individual is being formed. However, the questions of whether specific structures could arise depending on their parental origins or of their transcriptional status remain open. Our method chapter is dedicated to the technical description on how applying scHi-C to mouse embryos at different stages of preimplantation development. This approach capitalized with the limited amount of material available at these developmental stages. It also provides new research avenues, such as the study of mutant embryos for further functional studies.
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Acknowledgments
We thank Edith Heard for her strong scientific commitment and constant support during this project. We thank Takashi Nagano and Peter Fraser for their experimental expertise and technical support during the optimization of the Hi-C procedure on single mouse blastomeres. We thank the Institut Curie Animal facility for animal welfare and husbandry. This work was supported by FRM FDM20140630223 and FDM 40917 to N.R. and by funding allocated to Pr Edith Heard (Labex DEEP, ANR-11-LBX-0044; IDEX PSL, ANR-10-IDEX-0001-02 PSL).
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Ranisavljevic, N., Borensztein, M., Ancelin, K. (2021). Understanding Chromosome Structure During Early Mouse Development by a Single-Cell Hi-C Analysis. In: Ancelin, K., Borensztein, M. (eds) Epigenetic Reprogramming During Mouse Embryogenesis. Methods in Molecular Biology, vol 2214. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0958-3_19
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DOI: https://doi.org/10.1007/978-1-0716-0958-3_19
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