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Plant Chemical Genomics pp 131–144Cite as

Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors

Part of the Methods in Molecular Biology book series (MIMB,volume 2213)

Abstract

Auxin plays important roles in almost all aspects of plant growth and development. Chemical genetics is an effective approach to understand auxin action, especially in nonmodel plant species, in which auxin-related mutants are not yet available. Among auxin-related chemical tools, we present approaches to utilize auxin biosynthesis inhibitors. The inhibitors are effective not only to understand auxin biosynthesis but also to understand auxin action. The effectiveness of the inhibitors can be assessed based on in vitro or in vivo assays. The in vitro assay employs enzyme inhibition assays. The in vivo assay employs UPLC-MS/MS-based analysis of endogenous IAA and its intermediates or metabolites.

Key words

  • Chemical genetics
  • Arabidopsis
  • Auxin biosynthesis
  • Reverse genetics

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  • DOI: 10.1007/978-1-0716-0954-5_12
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Authors and Affiliations

  1. Western Region Agricultural Research Center (WARC), National Agriculture and Food Research Organization (NARO), Kagawa, Japan

    Kazuo Soeno

  2. Yokohama City University, Kihara Institute for Biological Research, Yokohama, Kanagawa, Japan

    Akiko Sato & Yukihisa Shimada

Authors
  1. Kazuo Soeno
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  2. Akiko Sato
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  3. Yukihisa Shimada
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Corresponding author

Correspondence to Yukihisa Shimada .

Editor information

Editors and Affiliations

  1. Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA, USA

    Glenn R. Hicks

  2. Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University, West Lafayette, IN, USA

    Chunhua Zhang

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Soeno, K., Sato, A., Shimada, Y. (2021). Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors. In: Hicks, G.R., Zhang, C. (eds) Plant Chemical Genomics. Methods in Molecular Biology, vol 2213. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0954-5_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0954-5_12

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