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Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening

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Plant Chemical Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2213))

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Abstract

The gaseous hormone ethylene regulates a diverse range of plant development and stress responses. Ethylene biosynthesis is tightly regulated by the transcriptional and posttranscriptional regulation of ethylene biosynthetic enzymes. ACC synthase (ACS) is the rate-limiting enzyme that controls the speed of ethylene biosynthesis in plant tissues, thus serving as a primary target for biotic and abiotic stresses to modulate ethylene production. Despite the critical role of ACS in ethylene biosynthesis, only a few regulatory components regulating ACS stability or ACS transcript levels have been identified and characterized. Here we show a genetic approach for identifying novel regulatory components in ethylene biosynthesis by screening EMS-mutagenized Arabidopsis seeds.

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Correspondence to Gyeong Mee Yoon .

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Park, C., Seo, D.H., Buckley, J., Yoon, G.M. (2021). Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening . In: Hicks, G.R., Zhang, C. (eds) Plant Chemical Genomics. Methods in Molecular Biology, vol 2213. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0954-5_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0954-5_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0953-8

  • Online ISBN: 978-1-0716-0954-5

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