Abstract
Since the recognition of the reactive oxygen species singlet oxygen (1O2) as a versatile signal that induces various stress responses, the mechanisms underlying 1O2-induced signaling transduction pathways have become the subject of much current research. This in turn highlights the need for reliable detection methods for 1O2. Here we describe a protocol for the detection of 1O2 using a commercially available fluorescent probe (Singlet Oxygen Sensor Green) and provide a simple method for direct visualization and quantification of the 1O2-evolving photosensitizer protochlorophyllide in the Arabidopsis fluorescent mutant.
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Acknowledgments
This work was supported by the Deutsche Forschungsgemeinschaft, TRR175, project C01 (to T.K.).
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Wang, L., Kleine, T. (2021). Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana. In: Espada, J. (eds) Reactive Oxygen Species. Methods in Molecular Biology, vol 2202. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0896-8_5
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DOI: https://doi.org/10.1007/978-1-0716-0896-8_5
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0896-8
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