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EPR Spectroscopy as a Method for ROS Quantification in the Skin

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Reactive Oxygen Species

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2202))

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.

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References

  1. Lohan SB, Muller R, Albrecht S, Mink K, Tscherch K, Ismaeel F, Lademann J, Rohn S, Meinke MC (2016) Free radicals induced by sunlight in different spectral regions - in vivo versus ex vivo study. Exp Dermatol 25:380–385

    Article  CAS  Google Scholar 

  2. Souza C, Maia Campos P, Schanzer S, Albrecht S, Lohan SB, Lademann J, Darvin ME, Meinke MC (2017) Radical-scavenging activity of a sunscreen enriched by antioxidants providing protection in the whole solar spectral range. Skin Pharmacol Physiol 30:81–89

    Article  CAS  Google Scholar 

  3. D’Orazio J, Jarrett S, Amaro-Ortiz A, Scott T (2013) UV radiation and the skin. Int J Mol Sci 14:12222–12248

    Article  CAS  Google Scholar 

  4. Zastrow L, Groth N, Klein F, Kockott D, Lademann J, Renneberg R, Ferrero L (2009) The missing link–light-induced (280–1,600 nm) free radical formation in human skin. Skin Pharmacol Physiol 22:31–44

    Article  CAS  Google Scholar 

  5. Herrling T, Fuchs J, Rehberg J, Groth N (2003) UV-induced free radicals in the skin detected by ESR spectroscopy and imaging using nitroxides. Free Radic Biol Med 35:59–67

    Article  CAS  Google Scholar 

  6. Kalyanaraman B, Darley-Usmar V, Davies KJ, Dennery PA, Forman HJ, Grisham MB, Mann GE, Moore K, Roberts LJ 2nd, Ischiropoulos H (2012) Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med 52:1–6

    Article  CAS  Google Scholar 

  7. Ahlberg S, Rancan F, Epple M, Loza K, Hoppe D, Lademann J, Vogt A, Kleuser B, Gerecke C, Meinke MC (2016) Comparison of different methods to study effects of silver nanoparticles on the pro- and antioxidant status of human keratinocytes and fibroblasts. Methods 109:55–63

    Article  CAS  Google Scholar 

  8. Meyer W, Schwarz R, Neurand K (1978) The skin of domestic mammals as a model for the human skin, with special reference to the domestic pig. Curr Probl Dermatol 7:39–52

    Article  CAS  Google Scholar 

  9. Jacobi U, Kaiser M, Toll R, Mangelsdorf S, Audring H, Otberg N, Sterry W, Lademann J (2007) Porcine ear skin: an in vitro model for human skin. Skin Res Technol 13:19–24

    Article  Google Scholar 

  10. Diffey BL, Farr PM (1989) The normal range in diagnostic phototesting. Br J Dermatol 120:517–524

    Article  CAS  Google Scholar 

  11. Albrecht S, Ahlberg S, Beckers I, Kockott D, Lademann J, Paul V, Zastrow L, Meinke MC (2016) Effects on detection of radical formation in skin due to solar irradiation measured by EPR spectroscopy. Methods 109:44–54

    Article  CAS  Google Scholar 

  12. Albrecht S, Elpelt A, Kasim C, Reble C, Mundhenk L, Pischon H, Hedtrich S, Witzel C, Lademann J, Zastrow L, Beckers I, Meinke MC (2019) Quantification and characterization of radical production in human, animal and 3D skin models during sun irradiation measured by EPR spectroscopy. Free Radic Biol Med 131:299–308

    Article  CAS  Google Scholar 

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Acknowledgments

This work was funded by the German Federal Ministry of Education and Research BMBF (Project number 03ZZ0140A) within the program “Twenty20—Partnership for Innovation” and carried out within the consortium “Advanced UV for Life.”

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Authors and Affiliations

  1. Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany

    Silke B. Lohan, Daniela Ivanov, Stephanie Albrecht & Martina C. Meinke

  2. Freiberg Instruments GmbH, Freiberg, Germany

    Nadine Schüler & Bastian Berger

Authors
  1. Silke B. Lohan
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  2. Daniela Ivanov
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  3. Nadine Schüler
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  4. Bastian Berger
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  5. Stephanie Albrecht
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  6. Martina C. Meinke
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Corresponding author

Correspondence to Martina C. Meinke .

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Editors and Affiliations

  1. Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O’Higgins, Santiago, Chile, Madrid, Spain

    Jesús Espada

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Lohan, S.B., Ivanov, D., Schüler, N., Berger, B., Albrecht, S., Meinke, M.C. (2021). EPR Spectroscopy as a Method for ROS Quantification in the Skin. In: Espada, J. (eds) Reactive Oxygen Species. Methods in Molecular Biology, vol 2202. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0896-8_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0896-8_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0895-1

  • Online ISBN: 978-1-0716-0896-8

  • eBook Packages: Springer Protocols

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