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Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe

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Yeast Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2196))

Abstract

Recent years have seen great progresses in third-generation sequencing. New commercial platforms from Oxford Nanopore Technologies (ONT) can generate ultra-long reads from single-molecule nucleic acid fragments of kilobases up to megabases, exceeding the limitation of short reads and dependency on template amplification suffered by the previous generation of sequencing technologies. Moreover, it can detect epigenetic modifications directly, as well as providing all-around field usage, being pocket-sized and low cost. It has already been applied to yeast research in many aspects, such as complete de novo genome assemblies, the phylogeny of large-brewing yeasts, gene isoform identification, and base modification detection. These applications have delivered novel insights into yeast genomic and transcriptomic analysis.

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Acknowledgments

This work is supported by funding from the Hundred-Talent Program of the Chinese Academy of Sciences (Y9CAS61107), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16010603), the National Natural Science Foundation of China (31970597), the National Key Research and Development Program of China (2019YFA0802202), and K.C. Wong Education Foundation (GJTD-2019-08). We thank Liuming Wang and Zihao Wang for their discussion and assistance in figure generation.

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Authors and Affiliations

  1. CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, China National Center for Bioinformation, Beijing, China

    Ming He, Xu Chi & Jie Ren

  2. University of Chinese Academy of Sciences, Beijing, China

    Ming He & Jie Ren

  3. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China

    Jie Ren

  4. Sino-Danish College, School of Future Technology, University of Chinese Academy of Sciences, Beijing, China

    Jie Ren

Authors
  1. Ming He
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  2. Xu Chi
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  3. Jie Ren
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Corresponding author

Correspondence to Jie Ren .

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Editors and Affiliations

  1. Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, College of Life Sciences, Capital Normal University Beijing, China, Saskatoon, SK, Canada

    Wei Xiao

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He, M., Chi, X., Ren, J. (2021). Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 2196. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0868-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0868-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0867-8

  • Online ISBN: 978-1-0716-0868-5

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