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Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE

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Yeast Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2196))

Abstract

Diversified genomes derived from chromosomal rearrangements are valuable materials for evolution. Naturally, chromosomal rearrangements occur at extremely low frequency to ensure genome stability. In the synthetic yeast genome project (Sc2.0), an inducible chromosome rearrangement system named Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is built to produce chromosomal rearrangements such as deletion, duplication, inversion, and translocation at high efficiency. Here, we detail the method to activate SCRaMbLE in a synthetic strain, to analyze the SCRaMbLEd genome, and to dissect the causative rearrangements for a desired phenotype after SCRaMbLEing.

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Acknowledgments

This work was supported by National Key Research and Development Program of China (2018YFA0900100), the National Natural Science Foundation of China (31725002, 31800069, and 31800082), Shenzhen Key Laboratory of Synthetic Genomics (ZDSYS201802061806209), Shenzhen Science and Technology Program (KQTD20180413181837372), Guangdong Provincial Key Laboratory of Synthetic Genomics (2019B030301006), and Bureau of International Cooperation, Chinese Academy of Sciences (172644KYSB20180022, 172644KYSB20170042).

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Authors and Affiliations

  1. CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China

    Zhouqing Luo, Shuangying Jiang & Junbiao Dai

Authors
  1. Zhouqing Luo
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  2. Shuangying Jiang
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  3. Junbiao Dai
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Corresponding author

Correspondence to Junbiao Dai .

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Editors and Affiliations

  1. Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, College of Life Sciences, Capital Normal University Beijing, China, Saskatoon, SK, Canada

    Wei Xiao

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Luo, Z., Jiang, S., Dai, J. (2021). Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 2196. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0868-5_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0868-5_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0867-8

  • Online ISBN: 978-1-0716-0868-5

  • eBook Packages: Springer Protocols

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