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Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics

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Metabolomics

Part of the book series: Neuromethods ((NM,volume 159))

Abstract

Multidimensional separation systems have arisen in the last years to overcome certain limitations of the classical one-dimensional separations. Multidimensional analytical approaches achieve a greater separation power, a crucial aspect when dealing with highly complex samples, as in metabolomics and lipidomics. Online comprehensive two-dimensional chromatography is a particularly interesting mode when pursuing untargeted analysis, which allows for separating, and consequently identifying a more extensive number of analytes. This chapter aims to summarize current applications of 2D-LC to the fields of metabolomics and lipidomics, and setups of different separation mechanism that are being employed, showing the most suitable combinations of chromatographic modes, depending on the target compounds.

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Acknowledgments

The research leading to these results has received funding from the Spanish Ministry of Science and Innovation (MCI, Grant CTQ2017-82598-P). MPC acknowledges a predoctoral FPU 16/02640 scholarship from Spanish Ministry of Education and Vocational Training (MEFP).

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Authors and Affiliations

  1. Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Spain

    Miriam Pérez-Cova, Romà Tauler & Joaquim Jaumot

Authors
  1. Miriam Pérez-Cova
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  2. Romà Tauler
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  3. Joaquim Jaumot
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Correspondence to Joaquim Jaumot .

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Editors and Affiliations

  1. Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN, USA

    Paul L. Wood

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Pérez-Cova, M., Tauler, R., Jaumot, J. (2021). Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics. In: Wood, P.L. (eds) Metabolomics . Neuromethods, vol 159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0864-7_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0864-7_3

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