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Untargeted Metabolomics Methods to Analyze Blood-Derived Samples

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Metabolomics

Part of the book series: Neuromethods ((NM,volume 159))

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Abstract

Untargeted metabolomics is applied to a wide range of preclinical and clinical studies. The metabolome is the furthest downstream product of the genome and is considered to provide a direct measure of the phenotype at the molecular level. Therefore, metabolomics seeks for the comprehensive detection and offers the broadest possible metabolome coverage in type of molecules and level of concentration. Nevertheless, the large number of molecules with physicochemical and structural diversity constituting the metabolome possess a bottleneck and is pointed as one of the greatest analytical challenges. Due to its high sensitivity and throughput, a commonly used mass spectrometry (MS) platform is often considered as a “gold standard” that plays a crucial role in untargeted metabolomics methods. Furthermore, the combination of separation techniques (LC, liquid chromatography; GC, gas chromatography; or CE, capillary electrophoresis) with mass spectrometry tremendously expands the capability of the analysis of highly complex biological samples with the highest metabolite coverage. This chapter exemplifies the strategies used for untargeted metabolomics analysis of blood derived samples. It gives experimental details on basic steps like blood plasma/serum withdrawal, metabolite extraction and analysis for LC-MS, GC-MS, and CE-MS untargeted metabolomic studies.

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Acknowledgments

The authors would like to express their gratitude to the financial support received from the Spanish Ministry of Science, Innovation and Universities (RTI2018-095166-B-I00) and Fondo Europeo de Desarrollo Regional (FEDER) and the Polish National Science Center (No 04-0225/09/529; DEC-2018/29/B/NZ7/02489). .

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Correspondence to Danuta Dudzik .

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Dudzik, D., García, A. (2021). Untargeted Metabolomics Methods to Analyze Blood-Derived Samples. In: Wood, P.L. (eds) Metabolomics . Neuromethods, vol 159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0864-7_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0864-7_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0863-0

  • Online ISBN: 978-1-0716-0864-7

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