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Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2191)

Abstract

Channelrhodopsin (ChR)-based optogenetics is one promising approach to restore vision in photoreceptor degenerative diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Currently, a large number of ChRs from different alga species as well as engineered variants are available. They vary with their light response properties like peak sensitive wavelength (λmax), current amplitude, and kinetics. Therefore, it is important to choose an appropriate ChR for practical applications, such as vision restoration. Here we describe a standard laboratory protocol for characterizing properties of ChRs in in vitro in human embryonic kidney (HEK) cells. Based on such characterization, we also discuss the criteria for selecting optimal ChRs for optogenetic vision restoration.

Key words

Channelrhodopsin (ChR) Optogenetics Vision restoration Operational light sensitivity HEK cells 
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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Department of Ophthalmology, Visual and Anatomical Sciences (OVAS)Wayne State University School of MedicineDetroitUSA

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