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An Introduction to Patch Clamp Recording

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Patch Clamp Electrophysiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2188))

Abstract

Electrophysiology is an essential tool aiding the study of the functions and dysfunctions of electrically excitable cells and their networks. The patch clamp method is a refined electrophysiological technique that can directly measure the membrane potential and/or the amount of current passing across the cell membrane. The patch clamp technique is also incredibly versatile and can be used in a variety of different configurations to study a range of properties, from spontaneous cell firing activity in native tissue to the activation and/or deactivation kinetics of individual channels expressed in recombinant cell lines. In this chapter we give an overview of patch clamping and how the different configurations can be set up and applied to electrophysiological research.

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Correspondence to Gary J. Stephens .

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Hill, C.L., Stephens, G.J. (2021). An Introduction to Patch Clamp Recording. In: Dallas, M., Bell, D. (eds) Patch Clamp Electrophysiology. Methods in Molecular Biology, vol 2188. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0818-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0818-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0817-3

  • Online ISBN: 978-1-0716-0818-0

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