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Mass Cytometry of Hematopoietic Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 2185)

Abstract

Mass cytometry is now a well-established method that enables the measurement of 40–50 markers (generally proteins but transcripts are also possible) in single cells. Analytes are detected via antibodies tagged with heavy metal and detected by using a time-of-flight mass spectrometer. Over the past decade, mass cytometry has proven to be a valuable method for immunophenotyping hematopoietic cells with remarkable precision in both healthy and malignant scenarios. This chapter explains in detail how to profile hematopoietic cells by using this high-dimensional multiplexed approach.

Key words

  • Mass cytometry
  • CyTOF
  • Hematopoiesis
  • Single-cell
  • High-dimensional
  • Multiplex
  • Immunophenotyping
  • Leukemia

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Correspondence to Kara L. Davis .

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Jager, A., Sarno, J., Davis, K.L. (2021). Mass Cytometry of Hematopoietic Cells. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

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