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Analysis and Isolation of Mouse Leukemic Stem Cells

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Part of the Methods in Molecular Biology book series (MIMB,volume 2185)

Abstract

Flow cytometry has been widely used in basic and clinical research for analysis of a variety of normal and malignant cells. Hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs) can be highly purified by flow cytometry. Isolated HSCs and LSCs can be functionally identified by transplantation assays and can also be studied at the molecular level. Here we describe the flow cytometry methods for analysis and isolation of mouse HSCs and LSCs.

Key words

  • Hematopoietic stem cells (HSCs)
  • Leukemic stem cells (LSCs )
  • Flow cytometry
  • Immunophenotype

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  • DOI: 10.1007/978-1-0716-0810-4_4
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Acknowledgments

This work was supported by the grant from the National Natural Science Foundation of China (81670105).

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Correspondence to Hideo Ema .

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Dong, F., Bai, H., Ema, H. (2021). Analysis and Isolation of Mouse Leukemic Stem Cells. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_4

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

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