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Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells

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Leukemia Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2185))

Abstract

Patient-derived induced pluripotent stem cells (iPSCs) have recently provided a new way to model acute myeloid leukemia (AML) and other myeloid malignancies. Here, we describe methods for the generation of patient-derived iPSCs from leukemia cells and for their subsequent directed in vitro differentiation into hematopoietic cells that recapitulate features of leukemia stem cells (LSCs) and leukemic blasts.

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Acknowledgments

The authors thank all members of the Papapetrou lab for their contributions to the development and refinement of the experimental protocols described here. This work was supported by NIH grants R01HL121570, R01HL137219, R01CA225231, and R01 HL132071 and by the Edward P. Evans Foundation, the New York State Stem Cell Science Board (NYSTEM), the Henry and Marilyn Taub Foundation, the Alex’s Lemonade Stand Foundation, the RUNX1 research program, the Pershing Square Sohn Cancer Research Alliance, and the Leukemia and Lymphoma Society.

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Authors and Affiliations

  1. Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    André G. Deslauriers, Andriana G. Kotini & Eirini P. Papapetrou

  2. Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    André G. Deslauriers, Andriana G. Kotini & Eirini P. Papapetrou

  3. Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    André G. Deslauriers, Andriana G. Kotini & Eirini P. Papapetrou

  4. Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    André G. Deslauriers, Andriana G. Kotini & Eirini P. Papapetrou

  5. Biotech Research and Innovation Center, University of Copenhagen, København, Denmark

    André G. Deslauriers

  6. Center for Hematologic Malignancies, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    André G. Deslauriers

Authors
  1. André G. Deslauriers
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  2. Andriana G. Kotini
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  3. Eirini P. Papapetrou
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Corresponding author

Correspondence to Eirini P. Papapetrou .

Editor information

Editors and Affiliations

  1. Immune System Development and Function Unit, Centro de Biologia Molecular "Severo Ochoa", (CSIC/UAM), Madrid, Spain

    César Cobaleda

  2. IBMCC, Laboratory 13, CSIC/Universidad de Salamanca, Salamanca, Spain

    Isidro Sánchez-García

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Deslauriers, A.G., Kotini, A.G., Papapetrou, E.P. (2021). Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_26

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_26

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

  • eBook Packages: Springer Protocols

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