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Isolation, Culture, and Manipulation of Human Cord Blood Progenitors

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Part of the Methods in Molecular Biology book series (MIMB,volume 2185)

Abstract

Umbilical Cord Blood (CB) is a rich source of hematopoietic stem/progenitor cells (HSPCs) with high proliferative capacity and a naïve immune status. These characteristics, among others, make CB a good source of HSPCs not only for transplantation, but also for biomedical research purposes. Here we describe the methods for human CB-HSPCs isolation, as well as their culture and cryopreservation, viral transduction and sorting, and in vivo and in vitro assays in order to study leukemic processes.

Key words

  • Hematopoietic progenitor cells
  • Sorting
  • Transduction
  • Clonogenic potential
  • Proliferation assay
  • Xenotransplantation
  • Engraftment

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  • DOI: 10.1007/978-1-0716-0810-4_17
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Correspondence to Cristina Prieto , Damia Romero-Moya or Rosa Montes .

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Prieto, C., Romero-Moya, D., Montes, R. (2021). Isolation, Culture, and Manipulation of Human Cord Blood Progenitors. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_17

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

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