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Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid

Part of the Methods in Molecular Biology book series (MIMB,volume 2185)

Abstract

Umbilical cord blood (UCB) units provide an alternative source of human hematopoietic stem cells (HSCs) for patients who require allogeneic stem cell transplantation but lack a matched donor. However, the limited number of HSCs within each UCB unit remains a major challenge for their use in regenerative medicine and HSC transplantation in adults. Efficient expansion of human HSCs in ex vivo cultures initiated with CD34+ cells isolated from UCBs can overcome this limitation. The method described here utilizes a deacetylase inhibitor, valproic acid (VPA), to rapidly expand to a high degree the numbers of functional HSCs and committed progenitors (HPCs). The expanded HSCs are capable of establishing both short-term and long-term multilineage hematopoietic reconstitution. This highly reproducible and simple protocol can be also applied to expansion of both HSCs and HPCs from different sources including the bone marrow and peripheral blood.

Key words

  • Ex vivo expansion
  • Valproic acid (VPA)
  • Hematopoietic stem cells (HSCs)
  • Umbilical cord blood (UCB)
  • Hematopoietic progenitor cells (HPCs)
  • Cryopreservation

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  • DOI: 10.1007/978-1-0716-0810-4_16
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References

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Correspondence to Luena Papa or Ronald Hoffman .

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Papa, L., Djedaini, M., Kintali, M., Schaniel, C., Hoffman, R. (2021). Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

  • eBook Packages: Springer Protocols