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Experimental Competitive Bone Marrow Transplant Assays

Part of the Methods in Molecular Biology book series (MIMB,volume 2185)

Abstract

Hematopoietic stem cells have the ability to produce all blood cells. When hematological malignancies occur, transplant of compatible blood or bone marrow cells from a healthy donor to the patient is an efficient solution to restore normal hematopoiesis. Bone marrow transplant in a mouse model is often used to study HSC function and capacity to repopulate an irradiated recipient. This protocol details the different steps of a competitive bone marrow transplant experiment, beginning with total body irradiation of the recipient mice; preparation and administration of the donor and competitor bone marrow samples; peripheral blood analysis to follow reconstitution posttransplant; and finally, the analysis of recipient bone marrow and secondary transplants to evaluate long-term HSC function. Different formulas used to establish transplant efficiency are explained. All the steps are discussed in detail, including tips, variations, and alternative procedures with their advantages and disadvantages.

Key words

  • Hematopoietic stem cells
  • Bone marrow transplantation
  • Total body irradiation
  • Flow cytometry
  • Blood collection
  • Mouse model
  • Competitive Repopulating Unit

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Acknowledgements

Roxann Hétu-Arbour and Sarah Bouali contributed equally to this work. Research in the lab was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC Discovery grant RGPIN-2018-05258), Canadian Institutes of Health Research (CIHR operating grant PJT-148614), and the Canada Foundation for Innovation (CFI Leaders Fund grant 31377). KMH is a Fonds de recherche du Québec – Santé (FRQS) Research Scholar. SB is supported by a CIHR Canada Graduate Scholarship.

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Correspondence to Krista M. Heinonen .

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Hétu-Arbour, R., Bouali, S., Heinonen, K.M. (2021). Experimental Competitive Bone Marrow Transplant Assays. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

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