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In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

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Leukemia Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2185))

Abstract

Leukemic stem cells are highly dynamic and heterogeneous. Analysis of leukemic stem cells at the single-cell level should provide a wealth of insights that would not be possible using bulk measurements. Mass spectrometry (MS)-based proteomic workflows can quantify hundreds or thousands of proteins from a biological sample and has proven invaluable for biomedical research, but samples comprising large numbers of cells are typically required due to limited sensitivity. Recent developments in sample processing, chromatographic separations, and MS instrumentation are now extending in-depth proteome profiling to single mammalian cells. Here, we describe specific techniques that increase the sensitivity of single-cell proteomics by orders of magnitude, enabling the promise of single-cell proteomics to become a reality. We anticipate such techniques can significantly advance the understanding of leukemic stem cells.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA

    Yiran Liang, Thy Truong & Ryan T. Kelly

  2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA

    Ying Zhu & Ryan T. Kelly

Authors
  1. Yiran Liang
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  2. Thy Truong
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  3. Ying Zhu
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  4. Ryan T. Kelly
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Corresponding author

Correspondence to Ryan T. Kelly .

Editor information

Editors and Affiliations

  1. Immune System Development and Function Unit, Centro de Biologia Molecular "Severo Ochoa", (CSIC/UAM), Madrid, Spain

    César Cobaleda

  2. IBMCC, Laboratory 13, CSIC/Universidad de Salamanca, Salamanca, Spain

    Isidro Sánchez-García

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Liang, Y., Truong, T., Zhu, Y., Kelly, R.T. (2021). In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics. In: Cobaleda, C., Sánchez-García, I. (eds) Leukemia Stem Cells. Methods in Molecular Biology, vol 2185. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0810-4_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0810-4_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0809-8

  • Online ISBN: 978-1-0716-0810-4

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