Abstract
Macrophages are professional innate immune cells that are broadly disseminated throughout the body, shape various innate and adaptive immune responses, and play crucial roles in inflammation, homeostasis, wound healing, and tissue remodelling. According to their surrounding microenvironments, macrophages can differentiate themselves in different phenotypes. Over the last two decades, gene expression profiling has been used to decipher new transcripts associated with macrophage phenotypes. This chapter outlines protocols used to isolate and culture murine macrophages and how they can be “polarized” to obtain a specific phenotype. Furthermore, we describe a protocol for gene expression profiling using a quantitative real-time polymerase chain reaction (qPCR), a high-standard technology in the field of gene expression.
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Acknowledgments
This chapter is supported by grants from the Canada Foundation for Innovation, Crohn’s and Colitis Canada, Research Manitoba, the Children’s Hospital Research Institute of Manitoba, the Natural Sciences and Engineering Research Council, and finally the Canadian Institutes of Health Research, to Jean-Eric Ghia. Nour Eissa is supported by the Canadian Institutes of Health Research (CIHR) (Grant# 395678), Children’s Hospital Research Institute of Manitoba, Health Science Centre Foundation (HSCF)-Mindel, and the Tom Olenick Research Excellence Award in Immunology and the MITACS Accelerate Program.
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Eissa, N., Hussein, H., Ghia, JE. (2020). A Gene Expression Analysis of M1 and M2 Polarized Macrophages. In: Mishra, S. (eds) Immunometabolism. Methods in Molecular Biology, vol 2184. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0802-9_10
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DOI: https://doi.org/10.1007/978-1-0716-0802-9_10
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