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Lipofection-Based Delivery of DNA Vaccines

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Vaccine Delivery Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2183))

Abstract

The preventive and therapeutic potential of DNA vaccines combined with benefits of lipid-based delivery (lipofection) allow efficient nucleic acid transfer and immunization applicable in treatment of infections, cancer or autoimmune disorders. Lipofecting compositions consisting of cationic and neutral lipids can be used for both in vitro and in vivo applications and may also play the role of adjuvants. Here we describe a simple protocol of DNA vaccine carrier preparation based on cationic polyprenyl derivatives (PTAI—trimethylpolyprenylammonium iodides) and commonly used helper lipids with use of basic laboratory equipment. Such formulas have proven effective for immunization of animals as well as for cell transfection.

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Acknowledgments

This chapter is dedicated to Professor Tadeusz Chojnacki of the Institute of Biochemistry and Biophysics, Polish Academy of Sciences (PAS) in Warsaw, the Father of Polish Polyprenology who started the cooperation between the Institute of Biochemistry and Biophysics PAS and Jagiellonian University. We would like to thank Professor Ewa Swiezewska of the Institute of Biochemistry and Biophysics PAS for inspiring cooperation, endless supplies of polyprenols, and critical reading of the manuscript. Preparation of cationic derivatives of polyprenols used in a series of projects, which contributed to this chapter, by Professor Marek Chmielewski and Doctor Marek Masnyk of the Institute of Organic Chemistry PAS is greatly appreciated. We also thank all the people from the Polish Vaccine Consortium (PVC) involved in the development of vaccines against influenza virus. This work was, in part, financially supported by the National Science Centre, Poland (2018/02/X/NZ3/01566 to M.R. and 2018/31/B/NZ3/01750 to Z.M.).

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Authors and Affiliations

  1. Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

    Monika Rak & Zbigniew Madeja

  2. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

    Anna Góra-Sochacka

Authors
  1. Monika Rak
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  2. Anna Góra-Sochacka
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  3. Zbigniew Madeja
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Corresponding author

Correspondence to Monika Rak .

Editor information

Editors and Affiliations

  1. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA

    Blaine A. Pfeifer

  2. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA

    Andrew Hill

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Rak, M., Góra-Sochacka, A., Madeja, Z. (2021). Lipofection-Based Delivery of DNA Vaccines. In: Pfeifer, B.A., Hill, A. (eds) Vaccine Delivery Technology. Methods in Molecular Biology, vol 2183. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0795-4_20

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  • DOI: https://doi.org/10.1007/978-1-0716-0795-4_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0794-7

  • Online ISBN: 978-1-0716-0795-4

  • eBook Packages: Springer Protocols

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