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Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells

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Vaccine Delivery Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2183))

Abstract

The discovery of the genome-editing tool CRISPR-Cas9 is revolutionizing the world of gene therapy and will extend the gene therapy product pipeline. While applying gene therapy products, the main difficulty is an efficient and effective transfer of the nucleic acids carrying the relevant information to their target destination, the nucleus of the cells. Baculoviruses have shown to be very suitable transport vehicles for this task due to, inter alia, their ability to transduce mammalian/human cells without being pathogenic. This property allows the usage of baculovirus-transduced cells as cell therapy products, thus, combining the advantages of gene and cell therapy. To make such pharmaceuticals available for patients, a successful production and purification is necessary. In this chapter, we describe the generation of a pseudotyped baculovirus vector, followed by downstream processing using depth and tangential-flow filtration. This vector is used subsequently to transduce human mesenchymal stem cells. The production of the cells and the subsequent transduction process are illustrated.

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Acknowledgments

F.E. was supported by the Heinrich Böll Foundation. The authors would like to thank the Hessen State Ministry of Higher Education, Research and the Arts for the financial support within the Hessen initiative for scientific and economic excellence (LOEWE-Program, LOEWE ZIB (Center for Insect Biotechnology and Bioresources). The authors acknowledge Catherine Meckel-Oschmann and Keven Lothert for revising the chapter.

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Authors and Affiliations

  1. Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany

    Friederike Eilts, Julie Harnischfeger, Daniel Loewe, Michael W. Wolff, Denise Salzig & Peter Czermak

  2. Faculty of Biology and Chemistry, Justus-Liebig-University Giessen, Giessen, Germany

    Peter Czermak

  3. Division Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, Germany

    Peter Czermak

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  1. Friederike Eilts
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  2. Julie Harnischfeger
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  3. Daniel Loewe
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  4. Michael W. Wolff
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  5. Denise Salzig
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  6. Peter Czermak
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Correspondence to Peter Czermak .

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  1. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA

    Blaine A. Pfeifer

  2. Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA

    Andrew Hill

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Eilts, F., Harnischfeger, J., Loewe, D., Wolff, M.W., Salzig, D., Czermak, P. (2021). Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells. In: Pfeifer, B.A., Hill, A. (eds) Vaccine Delivery Technology. Methods in Molecular Biology, vol 2183. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0795-4_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0795-4_19

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