Skip to main content
SpringerLink
Log in

Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research

  • Protocol
  • First Online:
Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2180))

Abstract

Genetic modifications in combination with highly sophisticated assisted reproductive technologies such as in vitro oocyte maturation and development, in vitro fertilization, intracytoplasmic sperm injection, and in vitro embryo culture have opened many research avenues and treatment options for both animals and humans. The number of genetically modified (GM) rodent strains increased considerably during the last several decades, and their numbers are expected to increase due to efficient gene editing technologies including the CRISPR/Cas9. Rodent ovarian tissues (OT) cryopreservation and transplantation procedures have several applications in biomedical field: they provide a fertility restoration option for GM rodent strains in some circumstances. They also serve as models to investigate OT cryopreservation as potential alternatives for human infertility patients as well as other domestic and wildlife species for the development of improved cryopreservation and subsequent transplantation strategies. The modeling studies enable determining effective cryoprotective agents (CPA), CPA and water permeability kinetics, and cooling and warming rates during the development of OT cryopreservation procedures. Furthermore, rodent models are extremely useful for determining post-thaw OT graft sites as well as potential medical interventions in an effort to expedite angiogenesis and inhibit inflammatory/immune response, OT longevity, and follicular integrity. Here we describe methodologies for rodent OT cryopreservation and potential transplantation sites for frozen-thawed rat and mouse OT.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Parkes AS, Smith AU (1954) Preservation of ovarian tissue at −79°C for transplantation. Acta Endocrinol 17:313–320

    CAS  Google Scholar 

  2. Deanesly R (1957) Egg survival in immature rat ovaries grafted after freezing and thawing. Proc R Soc Lond Ser B 147:412–421

    Article  CAS  Google Scholar 

  3. Green SH, Smith AU, Zuckerman S (1956) The number of oocytes in ovarian autografts after freezing and thawing. J Endocrinol 13:330–334

    Article  CAS  PubMed  Google Scholar 

  4. Robertson GAG (1940) Ovarian transplantation in the house mouse. Proc Soc Exp Biol Med 44:302–304

    Article  Google Scholar 

  5. Krohn PL (1958) Litters from CSH and CBA ovaries orthotopically transplanted into tolerant A strain mice. Nature 181:1671–1672

    Article  CAS  PubMed  Google Scholar 

  6. Parrott DVM (1959) Orthotopic ovarian grafts in the golden hamster. J Endocrinol 19:126–138

    Article  CAS  PubMed  Google Scholar 

  7. Parrott DVM (1960) The fertility of mice with orthotopic ovarian grafts derived from frozen tissue. J Reprod Fertil 1:230–241

    Article  Google Scholar 

  8. Agca Y (2000) Cryopreservation of oocytes and ovarian tissue. The National Academies, Institute for Lab. Anim Res J 41:207–220

    CAS  Google Scholar 

  9. Agca Y (2012) Genome resource banking of biomedically important laboratory animals. Theriogenology 78:1653–1665

    Article  PubMed  PubMed Central  Google Scholar 

  10. Harp R, Leibach J, Black J (1994) Cryopreservation of murine ovarian tissue. Cryobiology 31:336–343

    Article  CAS  PubMed  Google Scholar 

  11. Gunasena KT, Lakey JR, Villines PM, Critser ES, Critser JK (1997) Allogeneic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice. Biol Reprod 57:226–231

    Article  CAS  PubMed  Google Scholar 

  12. Sztein JM, McGregor TE, Bedigian HJ, Mobraaten LE (1999) Transgenic mouse strain rescue by frozen ovaries. Lab Anim Sci 49:99–100

    CAS  PubMed  Google Scholar 

  13. Takahashi E, Miyoshi I, Nagasu T (2001) Rescue of a transgenic mouse line by transplantation of a frozen-thawed ovary obtained postmortem. Contemp Top Lab Anim Sci 40:28–31

    CAS  PubMed  Google Scholar 

  14. Shaw JM, Trounson AO (2002) Ovarian tissue transplantation and cryopreservation. Application to maintenance and recovery of transgenic and inbred mouse lines. Methods Mol Biol 180:229–251

    PubMed  Google Scholar 

  15. Dorsch M, Wedekind D, Kamino K, Hedrich HJ (2007) Cryopreservation and orthotopic transplantation of rat ovaries as a means of gamete banking. Lab Anim 41:247–254

    Article  CAS  PubMed  Google Scholar 

  16. Dorsch M, Wedekind D, Kamino K, Hedrich HJ (2004) Orthotopic transplantation of rat ovaries as a tool for strain rescue. Lab Anim 38:307–312

    Article  CAS  PubMed  Google Scholar 

  17. Dawes J, Liu B, Mars W, Michalopoulos G, Khillan JS (2010) Multiple ovarian transplants to rescue a transgenic line of mice. Lab Anim (NY) 39:191–193

    Article  Google Scholar 

  18. Sztein JM (2011) Ovarian transplant for transgenic rescue. Lab Anim 40:49

    Article  Google Scholar 

  19. Ding Y, Shao JL, Li JW, Zhang Y, Hong KH, Hua KQ, Wang X (2018) Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model. J Ovarian Res 11:35

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  20. Ding Y, Shao J, Hua K, Wang X (2018) Modified model of whole ovary transplantation in rats. Transplant Proc 50:3881–3886

    Article  CAS  PubMed  Google Scholar 

  21. Salama M, Isachenko E, Ludwig S, Einzmann T, Rahimi G, Mallmann P, Isachenko VA (2019) Successful multidisciplinary approach for treatment and for preserving the reproductive potential in a rare case of acute lymphocytic leukemia during pregnancy. Gynecol Endocrinol 35:115–118

    Article  PubMed  Google Scholar 

  22. Cargill SL, Medrano JF, Anderson GB (1999) Infertility in a line of mice with the high growth mutation is due to luteal insufficiency resulting from disruption at the hypothalamic-pituitary axis. Biol Reprod 61:283–287

    Article  CAS  PubMed  Google Scholar 

  23. Torrents E, Boiso I, Barri PN, Veiga A (2003) Applications of ovarian tissue transplantation in experimental biology and medicine. Hum Reprod Update 9:471–481

    Article  CAS  PubMed  Google Scholar 

  24. Parker WH, Broder MS, Chang E, Feskanich D, Farquhar C, Liu Z, Shoupe D, Berek JS, Hankinson S, Manson JE (2009) Ovarian conservation at the time of hysterectomy and long-term health outcomes in the nurses’ health study. Obstet Gynecol 113:1027–1037

    Article  PubMed  PubMed Central  Google Scholar 

  25. Peterson RL, Parkinson KC, Mason JB (2017) Restoration of immune and renal function in aged females by re-establishment of active ovarian function. Reprod Fertil Dev 29:2052–2059

    Article  CAS  PubMed  Google Scholar 

  26. Anderson RA, Baird DT (2019) The development of ovarian tissue cryopreservation in Edinburgh: translation from a rodent model through validation in a large mammal and then into clinical practice. Acta Obstet Gynecol Scand 98:545–549

    Article  PubMed  Google Scholar 

  27. Silber S (2016) Ovarian tissue cryopreservation and transplantation: scientific implications. J Assist Reprod Genet 33:1595–1603

    Article  PubMed  PubMed Central  Google Scholar 

  28. Oktay K, Taylan E, Sugishita Y, Goldberg GM (2017) Robot-assisted laparoscopic transplantation of frozen-thawed ovarian tissue. J Minim Invasive Gynecol 24:897–898

    Article  PubMed  Google Scholar 

  29. Jensen AK, Macklon KT, Fedder J, Ernst E, Humaidan P, Andersen CY (2017) 86 successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children. J Assist Reprod Genet 34:325–336

    Article  PubMed  Google Scholar 

  30. Eppig JJ, O'Brien MJ (1996) Development in vitro of mouse oocytes from primordial follicles. Biol Reprod 54:197–207

    Article  CAS  PubMed  Google Scholar 

  31. Sztein JM, O'Brien MJ, Farley JS, Mobraaten LE, Eppig JJ (2000) Rescue of oocytes from antral follicles of cryopreserved mouse ovaries: competence to undergo maturation, embryogenesis, and development to term. Hum Reprod 15:567–571

    Article  CAS  PubMed  Google Scholar 

  32. Deng X, Zheng H, Yu X, Yu H, Zhang C, Chao L, Li R, Liu W (2009) Cryopreserved ovarian tissues can maintain a long-term function after heterotopic autotransplantation in rat. Reprod 138:519–525

    Article  CAS  Google Scholar 

  33. Agca C, Lucy M, Agca Y (2009) Gene expression profile of rat ovarian tissue following xeno transplantation into immune-deficient mice. Reproduction 137:957–967

    Article  CAS  PubMed  Google Scholar 

  34. Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y (2009) Orthotopic and heterotopic ovarian tissue transplantation. Hum Reprod Update 15:649–665

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Youm HY, Lee JR, Lee J, Jee BC, Suh CS, Kim SH (2015) Transplantation of mouse ovarian tissue: comparison of the transplantation sites. Theriogenology 83:854–861

    Article  PubMed  Google Scholar 

  36. Tavana S, Rezazadeh Valojerdi M, Eimani H, Abtahi NS, Fathi R (2017) Auto-transplantation of whole rat ovary in different transplantation sites. Vet Res Forum 8:275–280

    PubMed  PubMed Central  Google Scholar 

  37. Wietcovsky L, Til D, Salvador RA, Amaral NLL, Senn AP, Amaral VLL (2018) Ovarian tissue vitrification and heterotopic autologous transplantation in prepubertal Wistar rats. JBRA Assist Reprod 22:116–122

    PubMed  PubMed Central  Google Scholar 

  38. Mason JB, Cargill SL, Anderson GB, Carey JR (2009) Transplantation of young ovaries to old mice increased life span in transplant recipients. J Gerontol A Biol Sci Med Sci 64:1207–1211

    Article  PubMed  Google Scholar 

  39. Mason JB, Cargill SL, Griffey SM, Reader JR, Anderson GB, Carey JR (2011) Transplantation of young ovaries restored cardioprotective influence in postreproductive-aged mice. Aging Cell 10:448–456

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Mason JB, Terry BC, Merchant SS, Mason HM, Nazokkarmaher M (2015) Manipulation of ovarian function significantly influenced trabecular and cortical bone volume, architecture and density in mice at death. PLoS One 10:15

    Google Scholar 

  41. Bavister BD, Leibfried ML, Lieberman G (1983) Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 28:235–247

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors wish to thank The University of Missouri Mutant Mouse Resource and Research Center (NIH U42 OD010918-20; http://www.mu-mmrrc.com).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, University of Missouri, Columbia, MO, USA

    Yuksel Agca & Cansu Agca

Authors
  1. Yuksel Agca
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cansu Agca
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuksel Agca .

Editor information

Editors and Affiliations

  1. Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Agca, Y., Agca, C. (2021). Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research. In: Wolkers, W.F., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 2180. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0783-1_22

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0783-1_22

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0782-4

  • Online ISBN: 978-1-0716-0783-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation