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Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration

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The Epithelial-to Mesenchymal Transition

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2179))

Abstract

The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.

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Acknowledgments

We are in debt to Nicolas Daudet for his help and support. The Linker lab has been supported by grants from the Wellcome Trust, Royal Society, and the Medical Research council. SH and LB are funded by ICM P09-015-F, DAAD 57220037 & 57168868, CORFO 16CTTS-66390, Fondecyt 1181823, FONDEF 19I10334.

Author information

Authors and Affiliations

  1. Randall Centre for Cell and Molecular Biophysics, King’s College, London, UK

    Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Mint Ravinand Htun & Claudia Linker

  2. The Francis Crick Institute, London, UK

    Mint Ravinand Htun

  3. Department of Pediatrics Oncology and Hematology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt – Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

    Anton Gauert

  4. Program of Anatomy and Developmental Biology, BNI, CIMT, CENS, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile

    Luis Briones Montecinos & Steffen Härtel

Authors
  1. Zain Alhashem
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  2. Macarena Alvarez-Garcillan Portillo
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  3. Mint Ravinand Htun
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  4. Anton Gauert
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  5. Luis Briones Montecinos
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  6. Steffen Härtel
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  7. Claudia Linker
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Corresponding author

Correspondence to Claudia Linker .

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Editors and Affiliations

  1. Department of Biomedical Science and Bateson Centre, University of Sheffield, Sheffield, UK

    Kyra Campbell

  2. Centre de Biologie du Développement (CBD) Centre de Biologie Intégrative (CBI), Université de Toulouse, Toulouse, France

    Eric Theveneau

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Alhashem, Z. et al. (2021). Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration. In: Campbell, K., Theveneau, E. (eds) The Epithelial-to Mesenchymal Transition. Methods in Molecular Biology, vol 2179. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0779-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0779-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0778-7

  • Online ISBN: 978-1-0716-0779-4

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