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Aqueous Two-Phase Systems for Cleanup and Recovery of Enzymes from Plants and Plant-Derived Extracts

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Part of the Methods in Molecular Biology book series (MIMB,volume 2178)

Abstract

The increasing interest of the biopharmaceutical industry to exploit plants as a commercially viable production system is demanding the development of new strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological products, from organelles to proteins and low-molecular-weight compounds. The evaluation of different system parameters upon the partitioning behavior can provide the conditions that favor the concentration of contaminants and the desired target protein in opposite phases. The protocols described here provide the basic strategy to explore the use of ATPSs for the isolation and partial purification of native and recombinant proteins from plants and plant-derived extracts.

Key words

  • Aqueous two-phase systems
  • Plant proteins
  • Soybean
  • Sorghum
  • Green-tissue proteins

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  • DOI: 10.1007/978-1-0716-0775-6_7
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Acknowledgments

The authors wish to acknowledge the financial support from Tecnológico de Monterrey Bioprocesses Research Chair (Grant 0020209I13).

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Correspondence to Marco Rito-Palomares .

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Aguilar, O., Heredia-Olea, E., Perez-Carrillo, E., Rito-Palomares, M. (2021). Aqueous Two-Phase Systems for Cleanup and Recovery of Enzymes from Plants and Plant-Derived Extracts. In: Labrou, N.E. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 2178. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0775-6_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0775-6_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0774-9

  • Online ISBN: 978-1-0716-0775-6

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