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Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins

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Part of the Methods in Molecular Biology book series (MIMB,volume 2178)


Synthetic ligand affinity adsorbents offer an efficient means for purification of biopharmaceuticals. Single-isomer textile dye C.I. Reactive Blue and newer ligands developed by rational design and screening of chemical combinatorial libraries based on a triazine scaffold are routinely used for the capture and purification of these proteins from engineered recombinant expression systems. Here, we describe methods for the purification of recombinant human serum albumin and related fusion proteins using synthetic ligand affinity adsorbents.

Key words

  • Human serum albumin
  • Albumin fusion proteins
  • Affinity chromatography
  • Synthetic affinity adsorbents

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  • DOI: 10.1007/978-1-0716-0775-6_11
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Bastiaan Lobbezoo and Steve Burton for valuable discussions.

Prometic Bioseparations Ltd. is a trademark of Prometic Bioseparations Ltd. Mimetic Blue® is registered with the US Patent and Trademark Office. AlbuPure® and Albufuse® are registered trademarks of Albumedix Ltd. AlbuPure® is sold under license for the use in the purification of albumin fusion proteins with no warranty of merchantability or fitness for a particular purpose.

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Correspondence to Dev Baines .

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Williams, S., Morton, P., Baines, D. (2021). Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins. In: Labrou, N.E. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 2178. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0774-9

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