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In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance

Part of the Methods in Molecular Biology book series (MIMB,volume 2171)

Abstract

Human intestinal organoids (HIOs), derived from pluripotent stem cells, are a new tool to gain insights in gastrointestinal development, physiology, and associated diseases. Herein, we present a method for renal transplantation of HIOs in immunocompromised mice and subsequent analysis to study intestinal epithelial cell proliferation. In addition, we describe how to generate enteroids from transplanted HIOs. The method highlights the specific steps to successful engraftment and provides insight into the study of human intestinal stem cells.

Key words

  • Human intestinal organoids
  • Pluripotent stem cells
  • Transplantation
  • In vivo model
  • Intestinal stem cell
  • Enteroid

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  • DOI: 10.1007/978-1-0716-0747-3_12
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References

  1. Barker N (2014) Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration. Nat Rev Mol Cell Biol 15:19–33

    CAS  CrossRef  Google Scholar 

  2. Simons BD, Clevers H (2011) Stem cell self-renewal in intestinal crypt. Exp Cell Res 317:2719–2724

    CAS  CrossRef  Google Scholar 

  3. Noah TK, Donahue B, Shroyer NF (2011) Intestinal development and differentiation. Exp Cell Res 317:2702–2710

    CAS  CrossRef  Google Scholar 

  4. Date S, Sato T (2015) Mini-gut organoids: reconstitution of the stem cell niche. Annu Rev Cell Dev Biol 31:269–289

    CAS  CrossRef  Google Scholar 

  5. Spence JR, Mayhew CN, Rankin SA et al (2011) Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470:105–109

    CrossRef  Google Scholar 

  6. Watson CL, Mahe MM, Múnera J et al (2014) An in vivo model of human small intestine using pluripotent stem cells. Nat Med 20:1310–1314

    CAS  CrossRef  Google Scholar 

  7. McCracken KW, Howell JC, Wells JM, Spence JR (2011) Generating human intestinal tissue from pluripotent stem cells in vitro. Nat Protoc 6:1920–1928

    CAS  CrossRef  Google Scholar 

  8. Múnera JO, Wells JM (2017) Generation of gastrointestinal organoids from human pluripotent stem cells. Methods Mol Biol 1597:167–177

    CrossRef  Google Scholar 

  9. Mahe MM, Brown NE, Poling HM, Helmrath MA (2017) In vivo model of small intestine. Methods Mol Biol 1597:229–245

    CrossRef  Google Scholar 

  10. Mahe MM, Sundaram N, Watson CL et al (2015) Establishment of human epithelial enteroids and colonoids from whole tissue and biopsy. J Vis Exp (97):e52483. https://doi.org/10.3791/52483

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Correspondence to Maxime M. Mahe .

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Vales, S., Poling, H.M., Sundaram, N., Helmrath, M.A., Mahe, M.M. (2020). In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance. In: Ordóñez-Morán, P. (eds) Intestinal Stem Cells. Methods in Molecular Biology, vol 2171. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0747-3_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0747-3_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0746-6

  • Online ISBN: 978-1-0716-0747-3

  • eBook Packages: Springer Protocols