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Ribozymes pp 287-301 | Cite as

Long Non-coding RNA Depletion Using Self-Cleaving Ribozymes

  • Alex C. Tuck
  • Marc BühlerEmail author
Protocol
  • 189 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2167)

Abstract

Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription. We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.

Key words

Ribozyme RNA Genome editing lncRNA Long non-coding RNA lincRNA 

Notes

Acknowledgments

This work was supported by the Wellcome Trust [WT103977]; the Swiss National Science Foundation National Centres of Competence in Research RNA & Disease [141735]; and the Friedrich Miescher Institute for Biomedical Research, which is supported by the Novartis Research Foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Friedrich Miescher Institute for Biomedical ResearchBaselSwitzerland
  2. 2.University of BaselBaselSwitzerland

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