Long Non-coding RNA Depletion Using Self-Cleaving Ribozymes
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Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription. We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.
Key wordsRibozyme RNA Genome editing lncRNA Long non-coding RNA lincRNA
This work was supported by the Wellcome Trust [WT103977]; the Swiss National Science Foundation National Centres of Competence in Research RNA & Disease ; and the Friedrich Miescher Institute for Biomedical Research, which is supported by the Novartis Research Foundation.