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Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil

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Pollen and Pollen Tube Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2160))

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Abstract

As one of the essential steps to complete sexual reproduction, a pollen tube is precisely guided to an embryo sac to deliver the sperm cells. This ovule targeting by a pollen tube is one of the essential steps in pollen tube guidance. To assess the ovule targeting ability of the pollen tube from a certain mutant line, comparative analysis of pollen tube behaviors between wild-type and mutant genotypes is important. Here, we provide a protocol that traces all pollen tubes germinated from the quartet tetrad in a pistil by restricted pollination and aniline blue staining. By this analysis, statistical comparison between wild-type and the mutant pollen tube functions under the same in vivo condition is possible.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Number JP17H05832 (to T. I.), in addition to the NAGASE Science Technology Foundation (to T.I.) and the Strategic Priority Research Promotion Program on Phytochemical Plant Molecular Sciences, Chiba University.

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Authors and Affiliations

  1. Graduate School of Horticulture, Chiba University, Chiba, Japan

    Taro Takahashi & Tomoko Igawa

  2. Department of Tropical Medicine and Parasitology, Juntendo University, Tokyo, Japan

    Toshiyuki Mori

Authors
  1. Taro Takahashi
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  2. Toshiyuki Mori
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  3. Tomoko Igawa
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Corresponding author

Correspondence to Tomoko Igawa .

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Editors and Affiliations

  1. Department of Plant Science, McGill University, Montreal, QC, Canada

    Anja Geitmann

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Takahashi, T., Mori, T., Igawa, T. (2020). Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil. In: Geitmann, A. (eds) Pollen and Pollen Tube Biology. Methods in Molecular Biology, vol 2160. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0672-8_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0672-8_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0671-1

  • Online ISBN: 978-1-0716-0672-8

  • eBook Packages: Springer Protocols

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