Abstract
Myocardial infarction is a major clinical challenge for interventional, pharmacological, and potential molecular treatment of the ischemic insult. A large animal model with clinic-derived instrumentation allows for detailed imitation of interventional catheterization routines and application routes, whereas similar anatomy and heart proportions raise the possibility to precisely evaluate the efficacy of application modes, e.g., antegrade or retrograde intracoronary application of locally acting pharmaceutical agents, viruses, and cells. Here, we describe the techniques of left ventricular catheterization and induction of ischemia and reperfusion, as well as hemodynamic monitoring and regional application of therapeutic agents in pigs.
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References
Hinkel R et al (2017) Diabetes mellitus-induced microvascular destabilization in the myocardium. J Am Coll Cardiol 69(2):131–143
Längin M et al (2019) Consistent success in life-supporting porcine cardiac xenotransplantation. Nature 568(7752):E7
Kupatt C et al (2002) Retroinfusion of NFkappaB decoy oligonucleotide extends cardioprotection achieved by CD18 inhibition in a preclinical study of myocardial ischemia and retroinfusion in pigs. Gene Ther 9(8):518–526
Kupatt C et al (2005) Retroinfusion of embryonic endothelial progenitor cells attenuates ischemia-reperfusion injury in pigs: role of phosphatidylinositol 3-kinase/AKT kinase. Circulation 112(9_suppl):I-117–I-122
Hinkel R et al (2008) Thymosin ß 4 Is an essential paracrine factor of embryonic endothelial progenitor cell-mediated cardioprotection. Circulation 117(17):2232–2240
Hinkel R et al (2013) Inhibition of microRNA-92a protects against ischemia-reperfusion injury in a large animal model. Circulation 128(10):1066–1075
Hinkel R et al (2015) Heme oxygenase-1 gene therapy provides cardioprotection via control of post-ischemic inflammation: an experimental study in a pre-clinical pig model. J Am Coll Cardiol 66(2):154–165
Pleger ST et al (2011) Cardiac AAV9-S100A1 gene therapy rescues post-ischemic heart failure in a preclinical large animal model. Sci Transl Med 3(92):92ra64
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Bähr, A., Hornaschewitz, N., Kupatt, C. (2021). Myocardial Infarction in Pigs. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_4
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DOI: https://doi.org/10.1007/978-1-0716-0668-1_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0667-4
Online ISBN: 978-1-0716-0668-1
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