Abstract
Left ventricular catheterization in mice allows for in-depth assessment of myocardial function in healthy and diseased animals with the advent of pressure volume loop recordings greatly enhancing the technique. While a powerful tool, proper execution of the procedure is paramount to ensure reproducibility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance catheter system by Transonic; however, the basic method applies to all murine catheter systems. We furthermore indicate possible pitfalls during the procedure and how to avoid them.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Heinen A et al (2018) Echocardiographic analysis of cardiac function after infarction in mice: validation of single-plane long-axis view measurements and the bi-plane Simpson method. Ultrasound Med Biol 44(7):1544–1555
Lorenz JN, Robbins J (1997) Measurement of intraventricular pressure and cardiac performance in the intact closed-chest anesthetized mouse. Am J Phys 272(3 Pt 2):H1137–H1146
Kottam A et al (2011) Novel approach to admittance to volume conversion for ventricular volume measurement. Conf Proc IEEE Eng Med Biol Soc 2011:2514–2517
Baan J et al (1981) Continuous stroke volume and cardiac output from intra-ventricular dimensions obtained with impedance catheter. Cardiovasc Res 15(6):328–334
Schmitz S et al (2016) Comparison of physiological parameters and anaesthesia specific observations during isoflurane, ketamine-xylazine or medetomidine-midazolam-fentanyl anaesthesia in male guinea pigs. PLoS One 11(9):e0161258
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Ziegler, T., Laugwitz, KL., Kupatt, C. (2021). Left Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_3
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0668-1_3
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0667-4
Online ISBN: 978-1-0716-0668-1
eBook Packages: Springer Protocols