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Generation and Manipulation of Exosomes

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2158)

Abstract

Exosomes are membrane-bound nano-vehicles shed by most eukaryotic cells. Exosomes contain specific proteins and RNAs from parent cells, and they play key signaling roles in cellular development, modulation, and tissue regeneration. Attempts to isolate and modify exosomes to increase their targeting efficiency to specific tissue are still in their infancy. Here, we describe generation of exosomes from biopsy, isolation of exosomes by centrifugal ultrafiltration method, and approaches for manipulation of cardiac homing exosomes by chemical engineering for the treatment of myocardial infarction.

Key words

Exosome generation Exosome manipulation Myocardial infarction Cardiac homing peptide Platelet membrane Intravenous injection 
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Notes

Acknowledgments

This work was supported by grants from the National Institutes of Health to Dr. Ke Cheng (R01 HL123920, HL137093, HL144002, and HL146153) and the American Heart Association (18TPA34230092 and 19EIA34660286).

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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Department of Molecular Biomedical Sciences and Comparative Medicine InstituteNorth Carolina State UniversityRaleighUSA
  2. 2.Joint Department of Biomedical EngineeringUniversity of North Carolina at Chapel HillChapel HillUSA

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