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Apical Resection and Cryoinjury of Neonatal Mouse Heart

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Cardiac Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2158))

Abstract

Neonatal mouse hearts have a regenerative capacity similar to adult zebrafish. Different cardiac injury models have been established to investigate the regenerative capacity of neonatal mouse hearts, including ventricular amputation, cryoinjury, and ligation of a major coronary artery. While the ventricular resection model can be utilized to study how tissue forms and regenerates de novo, cryoinjury and coronary artery ligation are methods that might better mimic myocardial infarction by creating tissue damage and necrosis as opposed to the removal of healthy tissue in the ventricular amputation model. Here we describe methods of creating ventricular resection and cardiac cryoinjury in newborn mice.

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Author information

Authors and Affiliations

  1. Department of Stem Cell Biology and Regenerative Medicine, Broad CIRM Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Hua Shen & Henry M. Sucov

  2. Saban Research Institute and Heart Institute, Children’s Hospital Los Angeles, Los Angeles, CA, USA

    Ali Darehzereshki & Ching-Ling Lien

  3. Department of Surgery, MedStar Health Baltimore, Franklin Square Medical Center, Baltimore, MD, USA

    Ali Darehzereshki

  4. Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA

    Henry M. Sucov

  5. Division of Cardiology, Department of Medicine, Medical University of South Carolina, Charleston, SC, USA

    Henry M. Sucov

  6. Department of Surgery, Biochemistry and Molecular Medicine, University of Southern California, Los Angeles, CA, USA

    Ching-Ling Lien

Authors
  1. Hua Shen
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  2. Ali Darehzereshki
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  3. Henry M. Sucov
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  4. Ching-Ling Lien
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Corresponding author

Correspondence to Ching-Ling Lien .

Editor information

Editors and Affiliations

  1. Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

  2. Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, USA

    Bernhard Kühn

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Shen, H., Darehzereshki, A., Sucov, H.M., Lien, CL. (2021). Apical Resection and Cryoinjury of Neonatal Mouse Heart. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_2

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  • DOI: https://doi.org/10.1007/978-1-0716-0668-1_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0667-4

  • Online ISBN: 978-1-0716-0668-1

  • eBook Packages: Springer Protocols

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