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Ex Vivo Techniques to Study Heart Regeneration in Zebrafish

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Cardiac Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2158))

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Abstract

Due to its pronounced regenerative capacity, the zebrafish heart represents an advantageous model system for exploring the cellular and molecular mechanisms of cardiac regeneration. Upon injury, the epicardium, the outermost mesothelial tissue layer of vertebrate hearts, serves dual purposes in the regenerating heart as both a signaling center and a source for crucial cell types. Traditional in vivo genetic approaches to study heart regeneration can be time consuming and are not applicable to large-scale approaches and live surveillance of cellular behaviors. Here, we demonstrate ex vivo methods to culture, maintain, and study the regenerative responses of epicardial tissue in excised zebrafish hearts. Epicardial cell proliferation and migration are monitored in real time after uninjured or injured hearts are excised, washed, and cultured for up to 30 days. In addition to these techniques, we describe ex vivo genetic ablation of the epicardium, cell proliferation assays, partial ventricular explant culturing, and chemical screening.

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Acknowledgments

This work was supported by the American Heart Association Career Development Award (18CDA34110108) and Weill Cornell Start-up fund to J.C.

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Authors and Affiliations

  1. Department of Cell and Developmental Biology, Cardiovascular Research Institute, Weill Cornell Medical College, Cornell University, New York, NY, USA

    Sierra Duca & Jingli Cao

Authors
  1. Sierra Duca
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  2. Jingli Cao
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Corresponding author

Correspondence to Jingli Cao .

Editor information

Editors and Affiliations

  1. Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

  2. Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, USA

    Bernhard Kühn

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Duca, S., Cao, J. (2021). Ex Vivo Techniques to Study Heart Regeneration in Zebrafish. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0668-1_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0667-4

  • Online ISBN: 978-1-0716-0668-1

  • eBook Packages: Springer Protocols

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