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Identification of Long Noncoding RNA by In Situ Hybridization Approaches

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Molecular Dermatology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2154))

Abstract

In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

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Author information

Authors and Affiliations

  1. IDI-IRCCS, Rome, Italy

    Mara Mancini & Eleonora Candi

  2. Department of Experimental Medicine, University of Rome “Tor Vergata”, Rome, Italy

    Anna Maria Lena & Eleonora Candi

Authors
  1. Mara Mancini
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  2. Anna Maria Lena
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  3. Eleonora Candi
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Corresponding author

Correspondence to Eleonora Candi .

Editor information

Editors and Affiliations

  1. Monasterium Laboratory Skin and Hair Research Solutions GmbH, Münster, Germany

    ​Natalia V. Botchkareva

  2. Centre for Skin Sciences, University of Bradford, Bradford, West Yorkshire, UK

    Gillian E. Westgate

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Mancini, M., Lena, A.M., Candi, E. (2020). Identification of Long Noncoding RNA by In Situ Hybridization Approaches. In: Botchkareva, ​.V., Westgate, G.E. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 2154. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0648-3_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0648-3_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0647-6

  • Online ISBN: 978-1-0716-0648-3

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